Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11: XN6P4 ]
 
NCBI PubMed ID: 11956197
Journal NLM ID: 2985121R
Publisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Institutions: Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30303

We have identified and defined the function of kpsF of Neisseria meningitidis, and the homologues of kpsF in encapsulated K1 and K5 Escherichia coli. KpsF was shown to be the arabinose 5-phosphate isomerase, an enzyme not previously identified in prokaryotes that mediates the interconversion of ribulose 5-phosphate and arabinose 5- phosphate. KpsF is required for 3-deoxy-D-manno-octulosonic acid (Kdo) biosynthesis in N. meningitidis. Mutation of kpsF or the gene encoding the CMP-Kdo synthetase (kpsU/kdsB) in N. meningitidis resulted in expression of a lipooligossaccharide (LOS) structure that contained only lipid A and reduced capsule expression in the five invasive disease associated meningococcal serogroups (A, B, C, Y, and W-135). The step linking meningococcal capsule and LOS biosynthesis was shown to be Kdo production as the expression of capsule was wild type in a Kdo transferase (kdtA) mutant. Thus, in addition to lipooligosaccharide assembly, Kdo is required for meningococcal capsular polysaccharide expression. Further, N. meningitidis, unlike enteric gram-negative bacteria, can survive and synthesize only unglycosylated lipid A

biosynthesis, structure, Lipooligosaccharide, Neisseria meningitidis, disease, expression, gene, invasive, LOS, meningococcal, Neisseria, capsular, polysaccharide, Escherichia, Escherichia coli, acid, Kdo, transferase, lipid, lipid A, phosphate, capsular polysaccharide, type, wild type, infectious disease, bacteria, mutant, serogroup, assembly, production, reduced, medicine, Gram-negative bacteria, 3-deoxy-D-manno-octulosonic acid, arabinose, capsule, defined, enzyme, function, gram negative bacteria, Gram-negative, Infectious, interconversion, mutation, synthetase

Structure type: monomer
Location inside paper: Fig. 10
Contained glycoepitopes: IEDB_130650
 
Methods: biochemical methods
Biosynthesis and genetic data: biosynthesis data
 
Related record ID(s): 5408, 5409
NCBI Taxonomy refs (TaxIDs): 562
Reference(s) to other database(s): GTC:G70705ZI, GlycomeDB:37103
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