Hanniffy OM, Shashkov AS, Moran AP, Prendergast MM, Senchenkova SN, Knirel YA, Savage AV Chemical structure of a polysaccharide from Campylobacter jejuni 176.83 (serotype O:41) containing only furanose sugars Carbohydrate Research319(1-4) (1999)
124-132
The structure was elucidated in this paper NCBI PubMed ID:10520260 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: angela.savagenuigalway.ie Institutions: Department of Microbiology, National University of Ireland, Galway, Ireland, Department of Chemistry, National University of Ireland, Galway, Ireland, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow R- 17913, Russia
A neutral polysaccharide was obtained by hot phenol-water extraction of biomass from Campylobacter jejuni 176.83 and subsequently separated from acid-liberated core oligosaccharide of lipopolysaccharide by sequential GPC on Bio-Gel P6 and TSK-40 columns. All sugar components of the trisaccharide repeating unit of the polysaccharide were found to be of the furanose ring form. The major trisaccharide contained β-L-arabinose, 6-deoxy-β-D-altro-heptose (β-D-6d-altHep) and 6-deoxy-β-L-altrose (β-L-6d-Alt), whereas in the minor trisaccharide the β-L-6d-Alt is replaced by its C-5 epimer α-D-Fuc. On the basis of 1H and 13C NMR spectroscopic studies, including 2D ROESY, HMQC and HMQC-TOCSY experiments, the following structures of the repeating units were established: [formula: see text]
Methods: NMR-2D, NMR, acid hydrolysis Comments, role: major repeating unit of the polysaccharide has the structure: -2)bLAraf(1-2)bD6daltHepf(1-2)bL6dAltf(1- (75%) and minor repeating unit has the structure: -2)bLAraf(1-2)bD6daltHepf(1-2)aDFucf(1- (25%)
Related record ID(s): 478, 540, 542, 543 NCBI Taxonomy refs (TaxIDs):197 Reference(s) to other database(s): GTC:G62421AG, GlycomeDB:25246 Show glycosyltransferases
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