A polysaccharide containing D-Manp, L-Fucp (6-deoxygalactopyranose, fucose) and D-GlcpNAc was isolated by mild acid hydrolysis, followed by gel-permeation chromatography, from the lipopolysaccharide derived from Acinetobacter strain 96 (DNA group 11). The structure of the O-antigen was determined by compositional analysis and NMR spectroscopy of the polysaccharide as: [carbohydrate structure see text] A monoclonal antibody obtained after immunization of mice with heat-killed bacteria of Acinetobacter strain 96 was shown to bind to the O-antigen and did not cross-react with any Acinetobacter O-antigen of known structure.

Lipopolysaccharide, NMR, antigen, structure, blotting, chemistry, Bacterial, human, DNA, strain, structural, polysaccharide, O-antigen, analysis, O antigen, group, O-antigenic, O-antigenic polysaccharide, acid, Acinetobacter, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, animal, antigens, Carbohydrate Sequence, immunization, immunology, mice, Inbred BALB C, Molecular Sequence Data, epitopes, NMR spectroscopy, Cross Reactions, O antigens, O-antigens, bacteria, hydrolysis, serological, biological, Magnetic Resonance Spectroscopy, spectroscopy, gel permeation chromatography, Western, Carbohydrate Conformation, chromatography, classification, fucose, serotyping

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
4,3,3	aDManp	103.1	68.8	79.3	66.7	74.4	62.4
4,3	aLFucp	101.3	68.7	78.5	72.9	68.3	16.4
4,2	Ac	175.6	23.5
4	bDGlcpN	101.8	57.0	82.2	71.1	77.1	61.6
3	aLFucp	96.2	69.5	71.1	73.5	68.0	16.6
	bDManp	98.7	68.5	76.0	72.4	77.3	62.2


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
4,3,3	aDManp	5.18	4.27	4.16	3.86	3.84	?
4,3	aLFucp	5.06	3.91	3.97	3.98	4.35	1.22
4,2	Ac	-	2.08
4	bDGlcpN	4.58	3.83	3.74	3.40	3.51	3.69-4.02
3	aLFucp	5.10	3.87	4.10	3.92	4.65	1.30
	bDManp	4.89	4.30	3.85	4.06	3.51	3.80-3.97


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
4,3,3	aDManp	103.1/5.18	68.8/4.27	79.3/4.16	66.7/3.86	74.4/3.84	62.4/?
4,3	aLFucp	101.3/5.06	68.7/3.91	78.5/3.97	72.9/3.98	68.3/4.35	16.4/1.22
4,2	Ac		23.5/2.08
4	bDGlcpN	101.8/4.58	57.0/3.83	82.2/3.74	71.1/3.40	77.1/3.51	61.6/3.69-4.02
3	aLFucp	96.2/5.10	69.5/3.87	71.1/4.10	73.5/3.92	68.0/4.65	16.6/1.30
	bDManp	98.7/4.89	68.5/4.30	76.0/3.85	72.4/4.06	77.3/3.51	62.2/3.80-3.97

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

*O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O[C@H]2[C@H](O)[C@H](O[C@H]3[C@H](O)[C@@H](CO)O[C@@H](O[C@@H]4[C@@H](CO)O[C@@H](*)[C@@H](O)[C@H]4O[C@@H]4O[C@@H](C)[C@@H](O)[C@@H](O)[C@@H]4O)[C@@H]3NC(C)=O)O[C@@H](C)[C@H]2O)[C@H]1O

819.76 g/mol (C₃₂H₅₃R₂NO₂₃, R = next and previous repeats, not counted in mol weight)