Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 12702669Publication DOI: 10.1093/glycob/cwg074Journal NLM ID: 9104124Publisher: IRL Press at Oxford University Press
Correspondence: volpi
unimo.it
Institutions: Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213/d, 41100 Modena, Italy
Escherichia coli K4 bacterium synthesizes a nonsulfated capsule polysaccharide (K4) composed of a repeating disaccharide subunit of D-glucuronic acid (β1→3) and N-acetyl-D-galactosamine (β1→4) to which β-fructofuranose units are linked to C-3 of D-glucuronic acid residues. The K4 polyanion is easily defructosylated under acid conditions with no fragmentation of the polymer to produce a polysaccharide having a repeated disaccharide unit of chondroitin consisting of D-glucuronic acid (β1→3) and N-acetyl-D-galactosamine (β1→4) (K4d). K4 and K4d were depolymerized by partial digestion with testicular hyaluronidase and separated into uniform-size oligosaccharides from 4-mers to 16-mers by preparative anion-exchange chromatography after removal of the hyaluronidase. The purity and size of each oligosaccharide was confirmed by using anion-exchange HPLC, HPSEC analysis, and FACE. Mg-scale K4d oligosaccharides were obtained from 50 mg K4d starting material. Under the conditions used to degrade the K4 polysaccharide by testicular hyaluronidase, fructose is slowly liberated forming the defructosylated K4. As a consequence, a mixture of uniform- size K4 and K4d oligosaccharide species, from approximately 4- to 20-mers, are generated and size-separated by anion-exchange chromatography. These pure, uniform-size, and large ranges of K4d oligosaccharides having the structure of a chondroitin, →4)-GlcUA-β(1→3)GalNAc-β(1→, will be available for investigating important biological functions of this polymer.
Oligosaccharides, chondroitin, glycosaminoglycans, K4 polysaccharide
Structure type: polymer chemical repeating unit ; n=2-16,>16
Location inside paper: abstract
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_137473,IEDB_140630,IEDB_153510,IEDB_423153
Methods: FACE, HPLC, HPSEC
Enzymes that release or process the structure: testicular hyaluronidase
Related record ID(s): 5543, 6606, 6937, 8800, 9001, 9301, 10190
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G43702JT, GlycomeDB:
2738
Show glycosyltransferases
There is only one chemically distinct structure:
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