Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter [ICD11:
XN25B 
]
The structure was elucidated in this paperNCBI PubMed ID: 8774703Publication DOI: 10.1111/j.1432-1033.1995.899_3.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Division of Biochemical Microbiology, Center for Medicine and Biosciences, Research Center Borstel, Germany, Department of Medical Microbiology, Leiden University Hospital, Leiden, The Netherlands
Extraction of dry bacteria of Acinetobacter strain 34 (DNA group 2) or Acinetobacter strain 108 (DNA group 13) by phenol/water yielded a polymer that was identified by means of serological studies and fatty acid analysis as S-form lipopolysaccharide. Degradation of the lipopolysaccharides of strains 34 and 108 in 1% acetic acid and 5% acetic acid, respectively, and gel-permeation chromatography gave the respective O-antigenic polysaccharides, the structures of which were determined, by compositional analysis and NMR spectroscopy of the polysaccharide, as [Sequence: see text] for strain 108, where D-Fucp3NBuOH represents 3-[(R)-3-hydroxybutyramido] -3,6-dideoxy-D-galactose and D-GalpANAc represents 2-acetamido-2-deoxy-D-galacturonic acid. Both structures were specifically recognised in Western blots by polyclonal rabbit antisera and there was no cross-reaction between these two structures.
Lipopolysaccharide, NMR, Acinetobacter, serology, Western blot
Structure type: polymer chemical repeating unit
Location inside paper: abstract
Compound class: CPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_134624,IEDB_134627,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_141582,IEDB_141584,IEDB_141794,IEDB_147450,IEDB_151528,IEDB_153207,IEDB_153208,IEDB_190606,IEDB_742248,IEDB_885822,SB_163,SB_165,SB_166,SB_187,SB_195,SB_21,SB_23,SB_24,SB_25,SB_7,SB_8,SB_88
Methods: NMR-2D, NMR, composition analysis
Biological activity: serological data
Related record ID(s): 484, 5524, 5741, 5742
NCBI Taxonomy refs (TaxIDs): 472
Show glycosyltransferases
NMR conditions: in D2O at 335 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,4,2 Ac
6,4 bDGalpN 103.0 52.6 82.0 69.2 75.8 62.4
6,3,3,3,3 lR3HOBut 175.9 45.9 66.3 23.0
6,3,3,3 bDFucp3N 106.4 69.7 56.0 71.3 73.0 16.9
6,3,3,2 Ac
6,3,3 aDGalpN 94.7 49.5 78.4 70.1 72.6 62.2
6,3,2 Ac
6,3 bDGalpN 104.8 52.4 76.0 64.9 76.2 62.6
6 aDGalp 100.3 68.7 81.8 77.7 71.8 62.3
bDGalp 106.0 72.1 74.0 70.1 73.7 68.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,4,2 Ac
6,4 bDGalpN 4.87 4.00 3.82 4.10 3.53 ?
6,3,3,3,3 lR3HOBut - 2.47 4.17 1.23
6,3,3,3 bDFucp3N 4.48 3.48 3.91 3.68 3.81 1.23
6,3,3,2 Ac
6,3,3 aDGalpN 5.06 4.37 3.81 4.17 3.83 ?
6,3,2 Ac
6,3 bDGalpN 4.63 4.15 3.76 4.12 3.63 ?
6 aDGalp 4.90 3.73 3.83 4.31 3.85 ?
bDGalp 4.48 3.53 3.60 3.96 3.81 ?
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,4,2 Ac
6,4 bDGalpN 103.0/4.87 52.6/4.00 82.0/3.82 69.2/4.10 75.8/3.53 62.4/?
6,3,3,3,3 lR3HOBut 45.9/2.47 66.3/4.17 23.0/1.23
6,3,3,3 bDFucp3N 106.4/4.48 69.7/3.48 56.0/3.91 71.3/3.68 73.0/3.81 16.9/1.23
6,3,3,2 Ac
6,3,3 aDGalpN 94.7/5.06 49.5/4.37 78.4/3.81 70.1/4.17 72.6/3.83 62.2/?
6,3,2 Ac
6,3 bDGalpN 104.8/4.63 52.4/4.15 76.0/3.76 64.9/4.12 76.2/3.63 62.6/?
6 aDGalp 100.3/4.90 68.7/3.73 81.8/3.83 77.7/4.31 71.8/3.85 62.3/?
bDGalp 106.0/4.48 72.1/3.53 74.0/3.60 70.1/3.96 73.7/3.81 68.1/?
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,4,2 | Ac | |
| 6,4 | bDGalpN | 4.87 | 4.00 | 3.82 | 4.10 | 3.53 | ? |
| 6,3,3,3,3 | lR3HOBut |
| 2.47 | 4.17 | 1.23 | |
| 6,3,3,3 | bDFucp3N | 4.48 | 3.48 | 3.91 | 3.68 | 3.81 | 1.23 |
| 6,3,3,2 | Ac | |
| 6,3,3 | aDGalpN | 5.06 | 4.37 | 3.81 | 4.17 | 3.83 | ? |
| 6,3,2 | Ac | |
| 6,3 | bDGalpN | 4.63 | 4.15 | 3.76 | 4.12 | 3.63 | ? |
| 6 | aDGalp | 4.90 | 3.73 | 3.83 | 4.31 | 3.85 | ? |
| | bDGalp | 4.48 | 3.53 | 3.60 | 3.96 | 3.81 | ? |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,4,2 | Ac | |
| 6,4 | bDGalpN | 103.0 | 52.6 | 82.0 | 69.2 | 75.8 | 62.4 |
| 6,3,3,3,3 | lR3HOBut | 175.9 | 45.9 | 66.3 | 23.0 | |
| 6,3,3,3 | bDFucp3N | 106.4 | 69.7 | 56.0 | 71.3 | 73.0 | 16.9 |
| 6,3,3,2 | Ac | |
| 6,3,3 | aDGalpN | 94.7 | 49.5 | 78.4 | 70.1 | 72.6 | 62.2 |
| 6,3,2 | Ac | |
| 6,3 | bDGalpN | 104.8 | 52.4 | 76.0 | 64.9 | 76.2 | 62.6 |
| 6 | aDGalp | 100.3 | 68.7 | 81.8 | 77.7 | 71.8 | 62.3 |
| | bDGalp | 106.0 | 72.1 | 74.0 | 70.1 | 73.7 | 68.1 |
|
There is only one chemically distinct structure:
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