Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Acinetobacter baumannii [ICD11:
XN8LS ]
The structure was elucidated in this paperNCBI PubMed ID: 34537298Publication DOI: 10.1016/j.ijbiomac.2021.09.073Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: J.J. Kenyon <johanna.kenyon
qut.edu.au>
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology. Brisbane, Australia, School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, Australia
The KL26 gene cluster responsible for the synthesis of the K26 capsular polysaccharide (CPS) of Acinetobacter baumannii includes rmlBDAC genes for l-rhamnose (l-Rhap) synthesis, tle to generate 6-deoxy-l-talose (l-6dTalp) from l-Rhap, and a manC gene for D-mannose (D-Manp) that is rare in Acinetobacter CPS. K26 CPS material was isolated from A. baumannii isolate KZ-1098, and studied by sugar analysis, Smith degradation, and one and two-dimensional 1H and 13C NMR spectroscopy before and after O-deacetylation with aqueous ammonia. The following structure of the branched hexasaccharide repeating unit of the CPS was established: →2)-β-D-Manp-1→4-β-D-Glcp-1→3-α-L-6dTalp-1→3-β-D-GlcpNAc-(1→3↑14│Acα-L-Rhap-2←1-α-D-Glcp The structural depolymerase of phage vB_AbaP_APK26 cleaved selectively the β-GlcpNAc-(1→2)-α-Manp linkage in the K26 CPS formed by WzyK26 to give monomer, dimer, and trimer of the CPS repeating unit, which were characterized by high-resolution electrospray ionization mass spectrometry as well as 1H and 13C NMR spectroscopy. The wzyK26 gene responsible for this linkage and the manC gene were only found in six A. baumannii genomes carrying KL26 and one carrying the novel KL148 gene cluster, indicating the rare occurrence of β-GlcpNAc-(1→2)-α-Manp in A. baumannii CPS structures. However, K26 shares a β-d-Glcp-(1→3)-α-l-6dTalp-(1→3)-β-d-GlcpNAc trisaccharide fragment with a group of related A. baumannii CPSs that have varying patterns of acetylation of l-6dTalp.
Acinetobacter baumannii, capsular polysaccharide structure, 6-deoxy-L-talose, depolymerization, A.baumannii, phage depolymerase
Structure type: polymer chemical repeating unit
Location inside paper: table 1, p. 188, Fig. 6, DPS
Compound class: CPS
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_137340,IEDB_137485,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_225177,IEDB_885823,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, sugar analysis, GLC, Smith degradation, de-O-acetylation, HPLC, GPC, bioinformatic analysis, HR-ESI-MS, sequencing, phage characterization, depolymerization by phage
Enzymes that release or process the structure: (Gtr53,Gtr54),Wzy(K266), Gtr28, Atr6,Gtr29, Wzy(K266)[ItrA3]
Comments, role: O-deacetylated CPS
Related record ID(s): 5850, 5851, 5852, 5853, 5854, 5855, 5856, 5857, 10875
NCBI Taxonomy refs (TaxIDs): 470
Show glycosyltransferases
NMR conditions: in D2O at 328 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,4,3,2 aDGlcp 99.0 72.7 74.1 70.9 73.3 61.9
3,3,4,3 aLRhap 95.2 77.8 70.8 73.2 70.4 17.8
3,3,4 bDManp 102.5 72.5 77.1 66.6 77.8 62.8
3,3 bDGlcp 102.7 74.2 75.4 81.3 75.7 61.6
3 aL6dTalp 103.0 71.3 75.3 71.3 68.8 16.8
2 Ac 175.3 23.7
bDGlcpN 101.2 56.9 82.8 69.9 77.4 62.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,4,3,2 aDGlcp 5.00 3.57 3.79 3.45 4.05 3.80-3.85
3,3,4,3 aLRhap 5.12 4.03 3.97 3.54 3.93 1.29
3,3,4 bDManp 4.76 4.55 3.76 3.57 3.49 3.66-3.98
3,3 bDGlcp 4.69 3.46 3.79 3.66 3.64 3.78-3.90
3 aL6dTalp 5.06 3.93 4.03 3.93 4.30 1.24
2 Ac - 2.09
bDGlcpN 4.88 3.84 3.67 3.52 3.59 3.77-3.95
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,4,3,2 aDGlcp 99.0/5.00 72.7/3.57 74.1/3.79 70.9/3.45 73.3/4.05 61.9/3.80-3.85
3,3,4,3 aLRhap 95.2/5.12 77.8/4.03 70.8/3.97 73.2/3.54 70.4/3.93 17.8/1.29
3,3,4 bDManp 102.5/4.76 72.5/4.55 77.1/3.76 66.6/3.57 77.8/3.49 62.8/3.66-3.98
3,3 bDGlcp 102.7/4.69 74.2/3.46 75.4/3.79 81.3/3.66 75.7/3.64 61.6/3.78-3.90
3 aL6dTalp 103.0/5.06 71.3/3.93 75.3/4.03 71.3/3.93 68.8/4.30 16.8/1.24
2 Ac 23.7/2.09
bDGlcpN 101.2/4.88 56.9/3.84 82.8/3.67 69.9/3.52 77.4/3.59 62.2/3.77-3.95
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,4,3,2 | aDGlcp | 5.00 | 3.57 | 3.79 | 3.45 | 4.05 | 3.80
3.85 |
| 3,3,4,3 | aLRhap | 5.12 | 4.03 | 3.97 | 3.54 | 3.93 | 1.29 |
| 3,3,4 | bDManp | 4.76 | 4.55 | 3.76 | 3.57 | 3.49 | 3.66
3.98 |
| 3,3 | bDGlcp | 4.69 | 3.46 | 3.79 | 3.66 | 3.64 | 3.78
3.90 |
| 3 | aL6dTalp | 5.06 | 3.93 | 4.03 | 3.93 | 4.30 | 1.24 |
| 2 | Ac |
| 2.09 | |
| | bDGlcpN | 4.88 | 3.84 | 3.67 | 3.52 | 3.59 | 3.77
3.95 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,4,3,2 | aDGlcp | 99.0 | 72.7 | 74.1 | 70.9 | 73.3 | 61.9 |
| 3,3,4,3 | aLRhap | 95.2 | 77.8 | 70.8 | 73.2 | 70.4 | 17.8 |
| 3,3,4 | bDManp | 102.5 | 72.5 | 77.1 | 66.6 | 77.8 | 62.8 |
| 3,3 | bDGlcp | 102.7 | 74.2 | 75.4 | 81.3 | 75.7 | 61.6 |
| 3 | aL6dTalp | 103.0 | 71.3 | 75.3 | 71.3 | 68.8 | 16.8 |
| 2 | Ac | 175.3 | 23.7 | |
| | bDGlcpN | 101.2 | 56.9 | 82.8 | 69.9 | 77.4 | 62.2 |
|
There is only one chemically distinct structure:
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Found 1 record.
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