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1. (Article ID: 5887)
Alllen S, Richardson JM, Mehlert A, Ferguson MAJ
Structure of a complex phosphoglycan epitope from gp72 of Trypanosoma cruzi
Journal of Biological Chemistry 288(16) (2013)
11093-11095
The parasitic protozoan organism Trypanosoma cruzi is the causative agent of Chagas disease. The insect vector-dwelling epimastigote form of the organism expresses a low abundance glycoprotein associated with the flagellum adhesion zone, called gp72. The gp72 glycoprotein was first identified with an anti-carbohydrate IgG3 monoclonal antibody called WIC29.26 and has been shown to have an unusual sugar composition. Here, we describe a new way to isolate the WIC29.26 carbohydrate epitope of gp72. Using (1)H NMR and mass spectrometry before and after derivatization, we provide an almost complete primary chemical structure for the epitope, which is that of a complex phosphosaccharide: Galfb1-4Rhapa1-2Fucpa1-4(Galp?1-3)(Galpb1-2)Xylpb1-4Xylpb1-3(Xylpb1-2Galpa1-4(Galpb1-3)(Rhapa1-2)Fucpa1-4)GlcNAcp, with phosphate attached to one or other of the two Galp terminal residues and in which all residues are of the d-absolute configuration, except for fucose and rhamnose which are l. Combined with previous data (Haynes, P. A., Ferguson, M. A., and Cross, G. A. (1996) Glycobiology 6, 869-878), we postulate that this complex structure and its variants lacking one or more residues are linked to Thr and Ser residues in gp72 via a phosphodiester linkage (GlcNAcpa1-P-Thr/Ser) and that these units may form phosphosaccharide repeats through GlcNAcpa1-P-Galp linkages. The gp72 glycoprotein is associated with the flagellum adhesion zone on the parasite surface, and its ligation has been implicated in inhibiting parasite differentiation from the epimastigote to the metacyclic trypomastigote stage. The detailed structure of the unique phosphosaccharide component of gp72 reported here provides a template for future biosynthetic and functional studies.
epitope, mass spectrometry, Phosphoglycan, Trypanosoma cruzi
NCBI PubMed ID: 23436655Publication DOI: 10.1074/jbc.M113.452763Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: M.A.J .Ferguson
dundee.ac.uk>
Institutions: Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
Methods: 1H NMR, methylation, NMR-2D, GC-MS, SDS-PAGE, ESI-MS, mild acid hydrolysis, ESI-MS/MS, Western blotting, composition analysis, enzymatic digestion, HF treatment, HPTLC, butanolysis
The publication contains the following compound(s):
- Compound ID: 15090
|
a-L-Rhap-(1-2)-+
|
b-D-Xylp-(1-2)-a-D-Galp-(1-4)-a-L-Fucp-(1-4)-+
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b-D-Galp-(1-3)-+ |
|
a-D-Galp-(1-2)-+ |
| |
b-D-Galf-(1-4)-a-L-Rhap-(1-2)-a-L-Fucp-(1-4)-b-D-Xylp-(1-4)-b-D-Xylp-(1-3)-a-D-GlcpNAc
|
b-D-Galp-(1-3)-+ |
Show graphically |
Structure type: oligomer
Trivial name: phosphoglycan epitope of gp72 glycoprotein
Compound class: phosphoglycan
- Compound ID: 15091
|
a-L-Rhap-(1-2)-+
|
b-D-Xylp-(1-2)-a-D-Galp-(1-4)-a-L-Fucp-(1-4)-+
| |
b-D-Galp-(1-3)-+ |
|
a-D-Galp-(1-2)-+ |
| |
b-D-Galf-(1-4)-a-L-Rhap-(1-2)-a-L-Fucp-(1-4)-b-D-Xylp-(1-4)-b-D-Xylp-(1-3)-b-D-GlcpNAc
|
b-D-Galp-(1-3)-+ |
Show graphically |
Structure type: oligomer
Trivial name: phosphoglycan epitope of gp72 glycoprotein
Compound class: phosphoglycan
- Compound ID: 15092
|
a-L-Rhap-(1-2)-+
|
b-D-Xylp-(1-2)-a-D-Galp-(1-4)-a-L-Fucp-(1-4)-+
| |
b-D-Galp-(1-3)-+ |
|
?%P-?)-a-D-Galp-(1-2)-+ |
| |
b-D-Galf-(1-4)-a-L-Rhap-(1-2)-a-L-Fucp-(1-4)-b-D-Xylp-(1-4)-b-D-Xylp-(1-3)-D-GlcpNAc
|
?%P-?)-b-D-Galp-(1-3)-+ |
Show graphically |
Structure type: oligomer
Trivial name: phosphoglycan epitope of gp72 glycoprotein
Compound class: phosphoglycan
- Compound ID: 15093
|
a-L-Rhap-(1-2)-+
|
b-D-Xylp-(1-2)-a-D-Galp-(1-4)-a-L-Fucp-(1-4)-+
| |
b-D-Galp-(1-3)-+ |
|
a-D-Galp-(1-2)-+ |
| |
b-D-Galf-(1-4)-a-L-Rhap-(1-2)-a-L-Fucp-(1-4)-b-D-Xylp-(1-4)-b-D-Xylp-(1-3)-a-D-GlcpNAc-(1---P---/Thr/Ser/
|
b-D-Galp-(1-3)-+ |
Show graphically |
Structure type: structural motif or average structure
Aglycon: Thr/Ser
Trivial name: phosphosaccharide unit on gp72 glycoprotein
Compound class: phosphoglycan
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