Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Publication DOI: 10.1007/s10658-014-0424-7Journal NLM ID: 100955210Correspondence: Brigitte.mauch
unine.ch
Institutions: Laboratory of Molecular and Cellular Biology, Institute of Botany, University of Neuchâtel, Rue Emile-Argand 11, Case Postale 158, 2009, Neuchâtel, Switzerland, Metabolic Integration and Cell Signaling Group, Plant Physiology Section, Department of CAMN, Universitat Jaume I, Castellón, 12071, Spain, Laboratory for Fundamental and Applied Research in Chemical Ecology (FARCE), University of Neuchâtel, Neuchâtel, Switzerland, Root-Herbivore Interactions Group, Max Planck Institute for Chemical Ecology, Jena, Germany, Department of Biological Sciences, DePaul University, Chicago, IL, USA
The role of the salicylic acid (SA) glycosides SA 2-O-β-D-glucose (SAG), SA glucose ester (SGE) and the glycosyl transferases UGT74F1 and UGT74F2 in the establishment of basal resistance of Arabidopsis against Pseudomonas syringae pv tomato DC3000 (Pst) was investigated. Both mutants altered in the corresponding glycosyl transferases (ugt74f1 and ugt74f2) were affected in their basal resistance against Pst. The mutant ugt74f1 showed enhanced susceptibility, while ugt74f2 showed enhanced resistance against the same pathogen. Both mutants have to some extent, altered levels of SAG and SGE compared to wild type plants, however, in response to the infection, ugt74f2 accumulated higher levels of free SA until 24 hpi compared to wild type plants while ugt74f1 accumulated lower SA levels. These SA levels correlated well with reduced expression in PR1 and EDS1 in ugt74f1. In contrast, ugt74f2 has enhanced expression of Enhanced Disease Susceptibility 1 (EDS1) but a strong reduction in the expression of several jasmonate (JA)-dependent genes. Bacterial infection interfered with the expression of Fatty Acid Desaturase (FAD), Lipoxygenase2 (LOX2), carboxyl methyltransferase1 (BSMT1) and 9-cis-epoxycarotenoid dioxygenase (NCED3) genes in ugt74f1, thus promoting an antagonistic effect with SA-signalling and leading to enhanced bacterial growth. UGT74F2 might be a target for bacterial effectors since bacterial mutants affected in effector synthesis were impaired in inducing UGT74F2 expression. These results suggest that UGT74F2 negatively influences the accumulation of free SA, hence leading to an increased susceptibility due to reduced SA levels and increased expression of the JA and ABA markers LOX-2, FAD and NCED-3.
Pseudomonas syringae, salicylic acid, SA 2-O-β-D-glucose, SA glucose ester
Structure type: monomer
Location inside paper: Figure 6
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: biological assays, LC-MS, genetic manipulations
Biological activity: participates in resistance against Pseudomonas syringae pv tomato DC3000
Enzymes that release or process the structure: UGT74F2
Related record ID(s): 60845
NCBI Taxonomy refs (TaxIDs): 3702
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There is only one chemically distinct structure:
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