Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: leaf
Publication DOI: 10.1016/j.phytochem.2007.12.010Journal NLM ID: 0151434Publisher: Elsevier
Institutions: School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea, School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea, Department of Plant Science, Seoul National University, Seoul 151-742, Republic of Korea, Bio-MAX Institute, Seoul National University, Seoul 151-818, Republic of Korea, Division of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Republic of Korea, Department of Biology, Kyungpook National University, Daegu 702-701, Republic of Korea
We reported previously that a recombinant salicylic acid (SA) glucosyltransferase1 (AtSGT1) from Arabidopsis thaliana catalyzes the formation of both SA 2-O-β-d-glucoside (SAG) and the glucose ester of SA (SGE). Here, transgenic Arabidopsis plants overexpressing AtSGT1 have been constructed, and their phenotypes analyzed. Compared to wild-type plants, transgenic plants showed an increased susceptibility to Pseudomonas syringae and reduced the accumulation levels of both free SA and its glucosylated forms (SAG and SGE). On the other hand, the overexpression increased the levels of methyl salicylate (MeSA) and methyl salicylate 2-O-β-d-glucoside (MeSAG), and also induced SA carboxyl methyltransferase1 (AtBSMT1) expression, whose products catalyze the conversion of SA to MeSA. Our data indicate that reduced resistance by AtSGT1 overexpression results from a reduction in SA content, which is at least in part caused by increases in MeSAG and MeSA levels at the expense of SA. Our study also suggests that genetic manipulation of AtSGT1 can be utilized as an important regulatory tool for pathogen control.
Arabidopsis thaliana, plant disease resistance, salicylic Acid (SA), SA glucosyltransferase, SA conjugate, SA metabolite
Structure type: monomer
Location inside paper: Figure 1
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: biological assays, enzymatic assay, genetic manipulations
Related record ID(s): 60849, 60850
NCBI Taxonomy refs (TaxIDs): 3702
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