Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
The structure was elucidated in this paperPublication DOI: 10.1016/j.febslet.2007.01.060Journal NLM ID: 0155157Publisher: Elsevier
Correspondence: gershenzon
ice.mpg.de)
Institutions: Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll Strasse 8, D-07745 Jena, Germany, Research Group Mass Spectrometry, Max Planck Institute for Chemical Ecology, Hans-Knöll Strasse 8, D-07745 Jena, Germany
The major anthocyanin in A. thaliana is a cyanidin derivative modified by glycosylation as well as by the addition of three acyl moieties: malonyl, p-coumaroyl, and sinapoyl. We have isolated a member of the BAHD acyltransferase family which catalyzes this malonylation reaction by combining a reverse genetics approach with biochemical genomics. A mutant line containing a T-DNA insertion in At3g29590, the gene encoding the malonylating enzyme, is incapable of producing malonylated anthocyanins. Transgenic plants harboring an RNAi silencing cassette for At3g29590 demonstrate a positive correlation between reduction in the At3g29590 gene transcript and the decrease of malonylated anthocyanins. Transcript levels for both At3g29590 and the epistatic gene At4g14090, encoding 5-O-anthocyanin glucosyltransferase, increase in several plant lines as they accumulate anthocyanin pigments. Investigation of the heterologously expressed and purified malonylating enzyme showed that the activity is specific for malonyl-CoA and for anthocyanins with 5-O-glucosylation. The malonyl transfer itself occurs only to the 5-O-glucoside function, and not to any of the other sugar moieties present in A. thaliana anthocyanins. Hence, both in vivo and in vitro results define the activity of the At3g29590-encoded enzyme as an anthocyanin 5-O-glucoside-6″-O-malonyltransferase (At5MAT).
acyltransferase, Arabidopsis thaliana, anthocyanin, BAHD, malonyl coenzyme A
Structure type: oligomer
Location inside paper: Figure 2
Trivial name: A11
Contained glycoepitopes: IEDB_114701,IEDB_116879,IEDB_142488,IEDB_146664,IEDB_167188,IEDB_174332,IEDB_983931,SB_192
Methods: NMR, ESI-MS, biological assays, HPLC, enzymatic assay, genetic manipulations
Related record ID(s): 61105, 61106, 61107
NCBI Taxonomy refs (TaxIDs): 3702
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There is only one chemically distinct structure:
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