Taxonomic group: protista / Euglenozoa
(Phylum: Euglenozoa)
Host organism: Homo sapiens
Organ / tissue: cell surface,
Life stage: epimastigoteAssociated disease: Chagas disease [ICD11:
1F53 
, ICD11:
XN56V ];
infection due to Trypanosoma cruzi [ICD11:
XN56V ]
The structure was elucidated in this paperNCBI PubMed ID: 22764297Publication DOI: 10.4269/ajtmh.2012.12-0015Journal NLM ID: 0370507Publisher: Northbrook, IL: American Society of Tropical Medicine and Hygiene
Correspondence: rsoares
cpqrr.fiocruz.br
Institutions: Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil
The glycosylphosphatidylinositol (GPI)-anchored mucins of Trypanosoma cruzi trypomastigotes play an important immunomodulatory role during the course of Chagas disease. Here, some biological activities of tGPI-mucins from four T. cruzi isolates, including benznidazole-susceptible (BZS-Y), benznidazole-resistant (BZR-Y), CL, and Colombiana, were evaluated. GPI-mucins were able to differentially trigger the production of interleukin-12 and nitric oxide in BALB/c macrophages and modulate LLC-MK2 cell invasion. The significance of these variations was assessed after analysis of the terminal α-galactosyl residues. Enzymatic treatment with α-galactosidase indicated a differential expression of O-linked α-galactosyl residues among the strains, with higher expression of this sugar in BZS-Y and BZR-Y T. cruzi populations followed by Colombiana and CL. Unweighted pair group method analysis of the carbohydrate anchor profile and biological parameters allowed the clustering of two groups. One group includes Y and CL strains (T. cruzi II and VI), and the other group is represented by Colombiana strain (T. cruzi I).
macrophages, invasion, Trypanosoma cruzi, Glycosylphosphatidylinositol, nitric oxide, mucins, Chagas disease
Structure type: oligomer
Location inside paper: p. 94
Trivial name: mucin oligosaccharide
Compound class: O-glycan
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
Methods: SDS-PAGE, anion-exchange chromatography, deamination, FACE, extraction, statistical analysis, cytokine production, enzymatic treatment, macrophage activity assay, CL-ELISA
Related record ID(s): 6172, 6173, 6174, 6175, 6176, 6177, 7374, 8371
NCBI Taxonomy refs (TaxIDs): 5693,
353153
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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