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1. (Article ID: 6217)
 
Drobiazko AY, Kasimova AA, Evseev PV, Schneider MM, Klimuk EI, Shashkov AS, Dmitrenok AS, Chizhov AO, Slukin PV, Skryabin YP, Volozhantsev NV, Miroshnikov KA, Knirel YA, Popova AV
Capsule-Targeting Depolymerases Derived from Acinetobacter baumannii Prophage Regions
International Journal of Molecular Sciences 23(9) (2022) 4971
 

In this study, several different depolymerases encoded in the prophage regions of Acinetobacter baumannii genomes have been bioinformatically predicted and recombinantly produced. The identified depolymerases possessed multi-domain structures and were identical or closely homologous to various proteins encoded in other A. baumannii genomes. This means that prophage-derived depolymerases are widespread, and different bacterial genomes can be the source of proteins with polysaccharide-degrading activities. For two depolymerases, the specificity to capsular polysaccharides (CPSs) of A. baumannii belonging to K1 and K92 capsular types (K types) was determined. The data obtained showed that the prophage-derived depolymerases were glycosidases that cleaved the A. baumannii CPSs by the hydrolytic mechanism to yield monomers and oligomers of the K units. The recombinant proteins with established enzymatic activity significantly reduced the mortality of Galleria mellonella larvae infected with A. baumannii of K1 and K92 capsular types. Therefore, these enzymes can be considered as suitable candidates for the development of new antibacterials against corresponding A. baumannii K types.

Acinetobacter baumannii, capsular polysaccharide, glycosidase, capsular type, phage receptor-binding proteins, prophages, structural depolymerase

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