The structure of the specific capsular polysaccharide produced by Streptococcus pneumoniae type 22F (American type 22) was investigated by high-field 1H and 13C nuclear magnetic resonance spectroscopy, composition, methylation analysis, and periodate oxidation studies. The polysaccharide was found to be a high molecular weight acidic polymer composed of D-glucose, D-galactose, D-glucuronic acid, and L-rhamnose residues to form a regular repeating hexasaccharide unit having the structure [formula: see text] in which the β-L-rhamnopyranosyl residues were substituted by O-acetyl groups in 80% of the repeating units. The 1H and 13C nmr resonances of the O-deacetylated type 22F polysaccharide were completely assigned by application of two-dimensional homo- and heteronuclear chemical shift correlation techniques. Keywords: Streptococcuspneumoniae polysaccharide, NMR analysis.

NMR analysis, Streptococcus pneumoniae polysaccharide

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
2	bLRhap	100.14	71.68-71.83	73.52	72.87-73.01	72.87-73.01	17.49
	xDEry-ol	61.26	80.29	71.68-71.83	63.50


1H NMR data:
missing...

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

C[C@@H]1O[C@H](O[C@@H](CO)[C@H](O)CO)[C@H](O)[C@H](O)[C@H]1O

268.262 g/mol (C₁₀H₂₀O₈)