Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Sus scrofa
Associated disease: pleuropneumonia [ICD11:
CA40 
, ICD11:
CA40.Y ]
The structure was elucidated in this paperPublication DOI: 10.1139/v91-035Journal NLM ID: 0372705Publisher: National Research Council of Canada Canada
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario
The structure of the O-antigen of Actinobacillus pleuropneumoniae serotype 12 was determined by 1D and 2D NMR spectroscopic methods, methylation analysis, and partial hydrolytic degradation. The O-polysaccharide was shown to consist of a trisaccharide repeating unit having the structure [formula in text].
Lipopolysaccharide, antigen, polysaccharide, Actinobacillus pleuropneumoniae
Structure type: oligomer
Location inside paper: p. 221, structure 2, table 3
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_114704,IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_7
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, gel filtration, NMR-2D, sugar analysis, TLC, HF treatment
Comments, role: structure 2 was obtained from acid hydrolysis of O-antigen
Related record ID(s): 630, 112335
NCBI Taxonomy refs (TaxIDs): 754349Reference(s) to other database(s): GTC:G48521NE, GlycomeDB:
3478
Show glycosyltransferases
NMR conditions: in D2O at 300(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,6 aDGalp 99.5 69.5 70.4 70.3 71.8 62.0
6 bDGalf 108.6 82.0 77.8 84.0 70.8 69.5
xDGal-ol 64.1 70.9 70.1 70.1 70.3 69.3
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,6 | aDGalp | 99.5 | 69.5 | 70.4 | 70.3 | 71.8 | 62.0 |
| 6 | bDGalf | 108.6 | 82.0 | 77.8 | 84.0 | 70.8 | 69.5 |
| | xDGal-ol | 64.1 | 70.9 | 70.1 | 70.1 | 70.3 | 69.3 |
|
There is only one chemically distinct structure:
Found 
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(previously named: Haemophilus pleuropneumoniae 12 (NRCC 4267))