Taxonomic group: bacteria / Chlamydiae, Chlamydiae, Proteobacteria, Proteobacteria, Proteobacteria
(Phylum: Chlamydiae, Chlamydiae, Proteobacteria, Proteobacteria, Proteobacteria)
Associated disease: infection due to Chlamydia trachomatis [ICD11:
XN4Q4 
];
infection due to Escherichia coli [ICD11:
XN6P4 ]
NCBI PubMed ID: 10951204Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: wbrabetz
fz-borstel.de
Institutions: Division of Medical and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany, Divizion of Medical and Biochemical Microbiology, Research Centre Borstel, Centre for Medicine and Biosciences,Borstel,Germany
The waaA gene encoding the essential, lipopolysaccharide (LPS)-specific 3-deoxy-Dmanno-oct-2-ulosonic acid (Kdo) transferase was inactivated in the chromosome of a heptosyltransferase I and II deficient Escherichia coli K-12 strain by insertion of gene expression cassettes encoding the waaA genes of Chlamydia trachomatis, Chlamydophila pneumoniae or Chlamydophila psittaci. The three chlamydial Kdo transferases were able to complement the knockout mutation without changing the growth or multiplication behaviour. The LPS of the mutants were serologically and structurally characterized in comparison to the LPS of the parent strain using compositional analyses, high performance anion exchange chromatography, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and specific monoclonal antibodies. The data show that chlamydial Kdo transferases can replace in E. coli K-12 the host's Kdo transferase and retain the product specificities described in their natural background. In addition, we unequivocally proved that WaaA from C. psittaci transfers predominantly four Kdo residues to lipid A, forming a branched tetrasaccharide with the structure α-Kdo-(2→8)-[α-Kdo-(2→4)]-α-Kdo-(2→4)-α-Kdo.
Lipopolysaccharide, LPS, gene, Escherichia, Escherichia coli, 3-deoxy-D-manno-oct-2-ulosonic acid, acid, Kdo, transferase, transferases, Chlamydiaceae, genetic complementation, multifunctional 3-deoxy-D-manno-oct-2, product specificity, ulosonic acid transferases
Structure type: oligomer
Location inside paper: Fig. 5, I
Trivial name: core-lipid A carbohydrate backbone
Compound class: core oligosaccharide, LPS
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_135394,IEDB_141807,IEDB_150908,IEDB_151531
Biosynthesis and genetic data: genetic data
Related record ID(s): 2426, 2541, 4475, 5691, 6287, 6372, 6417, 6418, 25552, 124180
NCBI Taxonomy refs (TaxIDs): 813,
182082,
83333Reference(s) to other database(s): GlycomeDB:
8179, CCSD:
46733, CBank-STR:13726
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error