Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
]
NCBI PubMed ID: 33048281Publication DOI: 10.1007/s10719-020-09954-8Journal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Correspondence: Flaviana Di Lorenzo <flaviana.dilorenzo
unina.it>
Institutions: Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, Naples, 80126, Italy, Centre of Microbial Host Interactions, Institute of Technology Tallaght, Dublin, 24, Ireland, School of Biomolecular and Biomedical Sciences, University College Dublin, Belfield, Dublin, 4, Ireland
Pandoraea sp. is an emerging Gram-negative pathogen in cystic fibrosis causing severe and persistent inflammation and damage of the lungs. The molecular mechanisms underlying the high pathogenicity of Pandoraea species are still largely unknown. As Gram-negatives, Pandoraea sp. express lipopolysaccharides (LPS) whose recognition by the host immune system triggers an inflammatory response aimed at the bacterial eradication from the infected tissues. The degree of the inflammatory response strongly relies on the fine structure of the LPS and, in particular, of its glycolipid moiety, i.e. the lipid A. Here we report the structure of the lipid A isolated from the LPS of a chronic strain of P. pulmonicola (RL 8228), one of the most virulent identified so far among the Pandoraea species. Our data demonstrated that the examined chronic strain produces a smooth-type LPS with a complex mixture of hypoacylated lipid A species displaying, among other uncommon characteristics, the 2-hydroxylation of some of the acyl chains and the substitution by an additional glucosamine on one or both the phosphate groups.
Lipopolysaccharide, lipid A, cystic fibrosis, MALDI-TOF mass spectrometry, Structural characterization, Pandoraea
Structure type: polymer chemical repeating unit
Location inside paper: p.143
Compound class: O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_151531,IEDB_885822
Methods: GC-MS, SDS-PAGE, dephosphorylation, MS/MS, MALDI-TOF MS, composition analysis, partial delipidation
Related record ID(s): 32182
NCBI Taxonomy refs (TaxIDs): 93221
Show glycosyltransferases
There is only one chemically distinct structure:
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