Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 10930727Journal NLM ID: 8902526Publisher: Oxford University Press
Correspondence: jlam
uoguelph.ca
Institutions: Department of Microbiology, University of Guelph, Gueph, Canada
The O antigen unit of Pseudomonas aeruginosa serotype O5 is a complex trisaccharide containing 2-acetamido-3-acetiminido-2,3-dideoxy- L-D-mannuronic acid, 2-acetimido-3-acetimido-2,3-dideoxy-L-D-mannuronic acid, and 2-acetimido-2,6-deoxy-L-D-galactosamine. Specific knockout mutations in the putative UDP-D-N-acetylglucosamine (UDP-D-GlcNAc) epimerase gene, wbpI, or the putative UDP-D-Nacetylmannosamine dehydrogenase gene, wbpA, resulted in strains that no longer produced B-band lipopolysaccharide, confirming the essential roles of these genes in B-band O antigen synthesis. Despite approximately 50% similarity of wbpI and wbpA to the Escherichia coli genes wecB (rffE) and wecC (rffD) involved in enterobacterial common antigen synthesis, cross-complementation experiments were not successful. These results imply that the P. aeruginosa UDP-D-GlcNAc precursor may be di-N-acetylated prior to further modification, preventing the E. coli enzymes from recognizing it as a substrate.
Lipopolysaccharide, gene, B-band, Escherichia, Escherichia coli, WbpA, WbpI
Structure type: polymer chemical repeating unit
Location inside paper: Table 1
Trivial name: ECA, ECA LPS, enterobacterial common antigen (ECA)
Compound class: enterobacterial common antigen
Contained glycoepitopes: IEDB_137340,IEDB_141807,IEDB_151531
Methods: genetic methods
Biosynthesis and genetic data: genetic data
Related record ID(s): 4304, 4340, 6634, 6635, 6636, 6637, 20045, 23689, 123017
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G63996VH, GlycomeDB:
7812
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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