Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia pseudomallei [ICD11:
XN3LD 
];
infection due to Pseudomonas pseudomallei [ICD11:
XN8AA ]
NCBI PubMed ID: 9988483Publication DOI: 10.1046/j.1365-2958.1998.01139.xJournal NLM ID: 8712028Publisher: Blackwell Publishing
Correspondence: woods
acs.ucalgary.ca
Institutions: University 0f Calgary Health Sciences Centre,Calgary,Canada
Melioidosis, an infection caused by the gram-negative bacterial pathogen Burkholderia pseudomallei, is endemic in south-east Asia and northern Australia. Acute septicaemic melioidosis is a major cause of morbidity and mortality, especially in north-east Thailand. B. pseudomallei is highly resistant to the bactericidal activity of normal human serum (NHS), and we have found that B. pseudomallei 1026b multiplies in 10-30% NHS. We developed a simple screen for the identification of serum-sensitive mutants based on this novel phenotype. Approximately 1200 Tn5-OT182 mutants were screened, and three serum-sensitive mutants were identified. The type II O-antigenic polysaccharide (O-PS) moiety of lipopolysaccharide was not present in the serum-sensitive mutants. A representative serum-sensitive mutant, SRM117, was killed by the alternative pathway of complement and was less virulent than 1026b in three animal models of melioidosis. The Tn5-OT182 integrations in the serum-sensitive mutants were physically linked on the B. pseudomallei chromosome, and further genetic analysis of this locus revealed a cluster of 15 genes required for type II O-PS production. The proteins encoded by these genes were similar to proteins involved in bacterial polysaccharide biosynthesis. The results presented here demonstrate that type II O-PS is essential for B. pseudomallei serum resistance and virulence.
Lipopolysaccharide, virulence, Burkholderia, O-antigenic polysaccharide, Burkholderia pseudomallei, serum resistance, melioidosis
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Related record ID(s): 6577, 23437, 108536, 116823
NCBI Taxonomy refs (TaxIDs): 28450Reference(s) to other database(s): GTC:G00784ZG, GlycomeDB:
3464
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There is only one chemically distinct structure:
Found 
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(previously named: Pseudomonas pseudomallei)