Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: seed,
seedling
NCBI PubMed ID: 11144274Publication DOI: 10.1007/s004250000411Journal NLM ID: 1250576Publisher: Berlin, New York, Springer
Correspondence: dstrack
ipb-halle.de
Institutions: Leibniz-Institut für Pflanzenbiochemie, Abteilung Sekundärstoffwechsel, Halle (Saale), Germany
A cDNA encoding a UDP-glucose:sinapate glucosyltransferase (SGT) that catalyzes the formation of 1-O-sinapoylglucose, was isolated from cDNA libraries constructed from immature seeds and young seedlings of rape (Brassica napus L.). The open reading frame encoded a protein of 497 amino acids with a calculated molecular mass of 55,970 Da and an isoelectric point of 6.36. The enzyme, functionally expressed in Escherichia coli, exhibited broad substrate specificity, glucosylating sinapate, cinnamate, ferulate, 4-coumarate and caffeate. Indole-3-acetate, 4-hydroxybenzoate and salicylate were not conjugated. The amino acid sequence of the SGT exhibited a distinct sequence identity to putative indole-3-acetate glucosyltransferases from Arabidopsis thaliana and a limonoid glucosyltransferase from Citrus unshiu, indicating that SGT belongs to a distinct subgroup of glucosyltransferases that catalyze the formation of 1-O-acylglucosides (beta-acetal esters).
glucosyltransferase, phenylpropanoids, Brassicaceae, Brassica, hydroxycinnamates, sinapate
Structure type: monomer
Location inside paper: p. 884, Fig. 1
Compound class: glycoside, phenolic glycoside
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: DNA techniques, cloning, gene expression
Enzymes that release or process the structure: UDP-glucose:sinapate glucosyltransferase (SGT)
NCBI Taxonomy refs (TaxIDs): 3708Reference(s) to other database(s): GenDB:AF287143
Show glycosyltransferases
There is only one chemically distinct structure:
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