The structure of the O-antigenic part of the lipopolysaccharide (LPS) obtained from the verotoxin-producing Escherichia coli O171 has been determined. (1)H and (13)C NMR spectroscopy techniques in combination with component analysis were used to elucidate the O-antigen structure of O-deacylated LPS. Subsequent NMR analysis of the native LPS revealed acetylation at O-7/O-9 of the sialic acid residue. The sequence of sugars was determined by inter-residue correlations in (1)H,(1)H-NOESY and (1)H,(13)C-heteronuclear multiple-bond correlation spectra. The O-antigen is composed of pentasaccharide repeating units with one equivalent of O-acetyl groups distributed over two positions: Based on biosynthetic considerations, this should also be the biological repeating unit
Lipopolysaccharide, NMR, LPS, structure, biosynthetic, chemistry, clinical, correlation, structural, polysaccharide, O-antigen, repeating unit, analysis, O antigen, group, Escherichia, Escherichia coli, determination, O-antigenic, O-antigenic polysaccharide, structural determination, acid, NMR spectroscopy, biological, sugar, position, medicine, spectroscopy, sialic acid, sequence, pentasaccharide, sugars, component, O-deacylated, NMR analysis, acetylation, PDF, organic, bacteriology, native, O-acetyl, biological repeating unit, verotoxin-producing Escherichia coli
NCBI PubMed ID: 17182015Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden, Department of Laboratory Medicine, Division of Clinical Bacteriology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, GLC, composition analysis
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