Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Shigella dysenteriae [ICD11:
XN285 
]
NCBI PubMed ID: 30583222Publication DOI: 10.1016/j.molimm.2018.12.011Journal NLM ID: 7905289Publisher: Elsevier
Correspondence: N.V. Bovin <professorbovin
yandex.ru>
Institutions: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, Moscow, Russian Federation, Semiotik LLC, Moscow, Russian Federation, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky pr., Moscow, Russian Federation, School of Engineering, Computer & Mathematical Sciences, Auckland University of Technology, Auckland 1010, New Zealand
Sepharose matrix without immobilized ligands binds antibodies from human blood serum or immunoglobulin preparations. The eluted antibodies bind bacterial polysaccharides having no structural similarity to agarose (Sepharose is a cross-linked polysaccharide agarose) with a high affinity. It is concluded that the identified antibodies are capable of recognizing spatial rather than linear epitopes of bacterial polysaccharides. This side activity of Sepharose matrix should be taken into account in isolating target antibodies and other proteins from human blood.
antibodies, bacteria, polysaccharides, printed glycan array, Agarose, Sepharose
Structure type: polymer biological repeating unit
Location inside paper: p.67, table 2, Sh. dysenteriae 3
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_136044,IEDB_136095,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_21,SB_7,SB_88
Methods: serological methods, UV, affinity chromatography, antibody binding, glycan array analysis, microarray binding assays, serum analysis, isolation of antibodies
Related record ID(s): 694, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 717, 719, 996
NCBI Taxonomy refs (TaxIDs): 984896
Show glycosyltransferases
There is only one chemically distinct structure:
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