Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Cyprinus carpio
Associated disease: motile aeromonad septicemia (MAS)
NCBI PubMed ID: 31284525Publication DOI: 10.3390/md17070399Journal NLM ID: 101213729Publisher: Basel, Switzerland: Molecular Diversity Preservation International
Correspondence: aturska
hektor.umcs.lublin.pl
Institutions: Department of Genetics and Microbiology, Maria Curie-Skłodowska University in Lublin, Akademicka 19, 20-033 Lublin, Poland, Laboratory of General Microbiology, Department of Biology of Bacteria, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland, Department of Fish Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland
Amongst Aeromonas spp. strains that are pathogenic to fish in Polish aquacultures, serogroup O6 was one of the five most commonly identified immunotypes especially among carp isolates. Here, we report immunochemical studies of the lipopolysaccharide (LPS) including the O-specific polysaccharide (O-antigen) of A. veronii bv. sobria strain K557, serogroup O6, isolated from a common carp during an outbreak of motile aeromonad septicemia (MAS) on a Polish fish farm. The O-polysaccharide was obtained by mild acid degradation of the LPS and studied by chemical analyses, mass spectrometry, and 1H and 13C NMR spectroscopy. It was revealed that the O-antigen was composed of two O-polysaccharides, both containing a unique sugar 4-amino-4,6-dideoxy-L-mannose (N-acetyl-L-perosamine, L-Rhap4NAc). The following structures of the O-polysaccharides (O-PS 1 and O-PS 2) were established.
structure, O-antigen, NMR spectroscopy, O-polysaccharide, mass spectrometry, lipopolysaccharide (LPS), Aeromonas, immunospecificity, L-perosamine, Fish pathogen
Structure type: polymer chemical repeating unit
Location inside paper: O-PS 1, Aeromonas hydrophila JCM 3968 (O6a)
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_885822
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, SDS-PAGE, sugar analysis, ELISA, GLC, Western blotting, MALDI-TOF MS, serological methods, GPC
Related record ID(s): 721, 1002
NCBI Taxonomy refs (TaxIDs): 644Reference(s) to other database(s): GTC:G47352OD
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error