Taxonomic group: protista / Euglenozoa
(Phylum: Euglenozoa)
Host organism: (mammal)
Organ / tissue: cell surface,
Life stage: epimastigoteAssociated disease: Chagas disease [ICD11:
1F53 
, ICD11:
XN56V ];
infection due to Trypanosoma cruzi [ICD11:
XN56V ]
The structure was elucidated in this paperNCBI PubMed ID: 22738032Publication DOI: 10.1111/j.1365-3024.2012.01378.xJournal NLM ID: 7910948Publisher: Oxford: Wiley
Correspondence: vduschak
conicet.gov.ar
Institutions: Instituto Nacional de Parasitología Dr Mario Fatala Chaben, ANLIS-Malbrán, Ministerio de Salud de la Nación, Buenos Aires, Argentina, CIHIDECAR(CONICET), Depto de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Sulphoglycosphingolipids, present on the surface of diverse cells, participate in the regulation of various cellular events. However, little is known about the structure and the role of sulphoglycosphingolipids in trypanosomatids. Herein, sulphated dihexosylceramide structures - composed mainly of sphingosine as the long chain base acylated with stearic acid - have been determined for the first time in Trypanosoma cruzi epimastigotes by UV-MALDI-TOF-MS analysis. Interestingly, inhibition ELISA assays using cruzipain as antigen and polyclonal rabbit antibodies specific for cruzipain, the major cysteine proteinase of T. cruzi, or for its C-terminal domain, have demonstrated (i) that sulphate epitopes are shared between cruzipain and sulphatides of T. cruzi, (ii) that cross-reactivity maps to the C-terminal domain and (iii) the existence of other antigenic determinants in the glycolipidic structures. These features provide evidence that sulphate groups are antigenic in sulphate-containing parasite glycoconjugates. Furthermore, IgG2 antibody levels inversely correlate with disease severity in chronic Chagas disease patients, suggesting that IgG2 antibodies specific for sulphated epitopes might be associated with protective immunity and might be considered as potential surrogates of the course of chronic Chagas disease.
Trypanosoma cruzi, cruzipain antibodies, sulphate moieties, sulphatides, UV-MALDI-TOF-MS
Structure type: oligomer ; 1012.6 [M+HSO3+H]+
Location inside paper: p. 505, fig. 3
Compound class: glycosphingolipid
Contained glycoepitopes: IEDB_115136,IEDB_137339,IEDB_140630,IEDB_142488,IEDB_146664,IEDB_423153,IEDB_983931,SB_192,SB_5
Methods: methylation, TLC, ELISA, anion-exchange chromatography, methanolysis, statistical analysis, desulfation, immunization, UV-MALDI-TOF MS
Comments, role: epimastigote form of Trypanosoma cruzi. Proposed ceramide structure d20:1-C18:0, where C18:0 = 2-aminooctadecane-1,3-diol = SMILES O{1}C{2}C(N)C(O)CCCCCCCCCCCCCCC.
NCBI Taxonomy refs (TaxIDs): 5693
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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