CSDB: Search results

Taxonomic group: protista / Euglenozoa (Phylum: Euglenozoa)
Host organism: Homo sapiens
Organ / tissue: cell surface, Life stage: epimastigote
Associated disease: Chagas disease [ICD11: 1F53

, ICD11: XN56V

]; infection due to Trypanosoma cruzi [ICD11: XN56V

]

NCBI PubMed ID: 24920542
Publication DOI: 10.1002/cbic.201400069
Journal NLM ID: 100937360
Publisher: Weinheim, Germany: Wiley Interscience
Correspondence: carronal

usp.br; mdbaruff

fcfrp.usp.br
Institutions: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, USP, Av. Café S/N, CEP 14040-903, Ribeirão Preto, SP (Brazil)

This study describes the synthesis of glycopeptides NHAc[βGal]-(Thr)2 -[αGalNAc]-(Thr)2 -[αGlcNAc]-(Thr)2 Gly-OVA (1-OVA) and NHAc[βGal-αGalNAc]-(Thr)3 -[αLacNAc]-(Thr)3 -Gly-OVA (2-OVA) as mimetics of both T. cruzi and tumor mucin glycoproteins. These glycopeptides were obtained by solid-phase synthesis, which involved the prior preparation of the protected glycosyl amino acids αGlcNAc-ThrOH (3), αGalNAc-ThrOH (4), βGal-ThrOH (5), αLacNAc-ThrOH (6), and βGal-αGalNAc-ThrOH (7) through glycosylation reactions. Immunizations of mice with glycopeptides 1-OVA and 2-OVA induced high antibody titers (1:16 000), as verified by ELISA tests, whereas flow cytometry assays showed the capacity of the obtained anti-glycopeptides 1-OVA and 2-OVA antibodies to recognize both T. cruzi and MCF-7 tumor cells. In addition, antisera induced by glycopeptides 1-OVA and 2-OVA were also able to inhibit T. cruzi fibroblast cell invasion (70 %) and to induce antibody-mediated cellular cytotoxicity (ADCC) against MCF-7 cells, with 50 % reduction of cell viability

antibodies, glycopeptides, Trypanosoma cruzi, mucins, antitumor agents

Structure type: fragment of a bigger structure
Location inside paper: p.1496, scheme 1A, TcMUC
Trivial name: mucin glycoprotein TcMUC
Compound class: glycoprotein
Contained glycoepitopes: IEDB_136044,IEDB_137340,IEDB_137472,IEDB_140108,IEDB_141794,IEDB_141807,IEDB_151531,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_30,SB_7,SB_88

Methods: ELISA, chemical synthesis, flow cytometry analysis, cytotoxicity assay, inhibition assay of Trypanosoma cruzi cell invasion

NCBI Taxonomy refs (TaxIDs): 5693
Show glycosyltransferases

There are 32 chemically distinct structures. Please, select:

CC(=O)N[C@H]1[C@@H](O[C@@H](C)[C@@H](NC(=O)[C@H](NC(=O)[C@H](N)[C@H](C)O)[C@H](C)O)C(=O)N[C@@H](C(=O)N[C@@H](C(=O)O)[C@H](C)O)[C@H](C)O)O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize