Taxonomic group: protista / Euglenozoa
(Phylum: Euglenozoa)
Host organism: Homo sapiens
Associated disease: infection due to Leishmania [ICD11:
XN8JE 
];
visceral leishmaniasis [ICD11:
1F54.0 , ICD11:
XN8JE ];
cutaneous leishmaniasis [ICD11:
1F54.1 , ICD11:
XN8JE ];
mucocutaneous leishmaniasis [ICD11:
1F54.2 , ICD11:
XN8JE ]
The structure was elucidated in this paperNCBI PubMed ID: 29806570Publication DOI: 10.1017/S0031182018000720Journal NLM ID: 0401121Publisher: London, New York, Cambridge University Press
Correspondence: p.bates
lancaster.ac.u
Institutions: Laboratory of Molecular Biology, Department of Bioprocess Engineering and Biotechnology, Universidade Federal do Paraná, Avenida Coronel Francisco Heráclito dos Santos, 210 - Usina Piloto B, Curitiba, Paraná 81531-970, Brazil, Nucleus of Medical Education, Department of Community Health, Universidade Federal do Paraná, Rua Padre Camargo, 280 - 7th floor, Curitiba, Paraná 80060-240, Brazil, Division of Biomedical and Life Sciences, Faculty of Health and Medicine,Lancaster University, Lancaster, Lancashire, LA1 4YQ,UK
Oligosaccharides are broadly present on Leishmania cell surfaces. They can be useful for the leishmaniases diagnosis and also helpful in identifying new cell markers for the disease. The disaccharide Galα1-3Galβ is the immunodominant saccharide in Leishmania cell surface and is the unique non-reducing terminal glycosphingolipids structure recognized by anti-α-Gal. This study describes an enzyme-linked immunosorbent assay (ELISA) used to measure serum levels of anti-α-galactosyl (α-Gal) antibodies in patients with cutaneous leishmaniasis (CL). Optimal ELISA conditions were established and two neoglycoproteins (NGP) containing the Galα1-3Gal terminal fraction (Galα1-3Galβ1-4GlcNAc-HAS and Galα1-3Gal-HAS) and one Galα1-3Gal NGP analogue (Galα1-3Galβ1-3GlcNAc-HAS) were used as antigens. Means of anti-α-Gal antibody titres of CL patients were significantly higher (P < 0.05) than the healthy individuals for all NGPs tested. Sensitivity and specificity of all NGPs ranged from 62.2 to 78.4% and 58.3 to 96.7%, respectively. In conclusion, the NGPs can be used for CL diagnosis.
antigen, ELISA, saccharide, α-galactosyl, L.braziliensis
Structure type: fragment of a bigger structure
Location inside paper: abstract
Trivial name: linear B, α-Gal epitope
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_149558,IEDB_151528,IEDB_190606,IEDB_918314,SB_165,SB_166,SB_187,SB_195,SB_7,SB_87,SB_88
Methods: ELISA, antibody binding, statistical analysis
Comments, role: non-reducing terminal glycosphingolipids structure recognized by anti-α-Gal.
NCBI Taxonomy refs (TaxIDs): 5658Reference(s) to other database(s): GTC:G24432YB
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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