Soares RP, Torrecilhas AC, Assis RR, Rocha MN, Moura e Castro FA, Freitas GF, Murta SM, Santos SL, Marques AF, Almeida IC, Romanha AJ Intraspecies Variation in Trypanosoma cruzi GPI-Mucins: Biological Activities and Differential Expression of alpha-Galactosyl Residues American Journal of Tropical Medicine and Hygiene87(187) (2012)
96
a-D-Manp-(1-2)-+ Aep-(1-6)-+ /Variants 0/-+
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Subst-(1-2)-EtN-(1--P--6)--a-D-Manp-(1-2)-a-D-Manp-(1-6)-a-D-Manp-(1-4)-a-D-GlcpN-(1-6)-L-myoIno-(1--P--3)--Gro1ALK
/Variants 0/ is:
Ste-(1-2)-
OR (exclusively)
Pam-(1-2)-
Subst = protein (eGPI-mucin)
NCBI PubMed ID:22764297 Publication DOI:10.4269/ajtmh.2012.12-0015 Journal NLM ID:0370507 Publisher: Northbrook, IL: American Society of Tropical Medicine and Hygiene Correspondence: rsoarescpqrr.fiocruz.br Institutions: Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil
The glycosylphosphatidylinositol (GPI)-anchored mucins of Trypanosoma cruzi trypomastigotes play an important immunomodulatory role during the course of Chagas disease. Here, some biological activities of tGPI-mucins from four T. cruzi isolates, including benznidazole-susceptible (BZS-Y), benznidazole-resistant (BZR-Y), CL, and Colombiana, were evaluated. GPI-mucins were able to differentially trigger the production of interleukin-12 and nitric oxide in BALB/c macrophages and modulate LLC-MK2 cell invasion. The significance of these variations was assessed after analysis of the terminal α-galactosyl residues. Enzymatic treatment with α-galactosidase indicated a differential expression of O-linked α-galactosyl residues among the strains, with higher expression of this sugar in BZS-Y and BZR-Y T. cruzi populations followed by Colombiana and CL. Unweighted pair group method analysis of the carbohydrate anchor profile and biological parameters allowed the clustering of two groups. One group includes Y and CL strains (T. cruzi II and VI), and the other group is represented by Colombiana strain (T. cruzi I).
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