Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 9720240Journal NLM ID: 0043535Publisher: Elsevier
Institutions: School of Pharmaceutical Sciences, Rhodes University, Grahamstown 6140, South Africa, Department of Chemistry, Carlsberg Laboratory, Gamle Carlsbergvej 10, 2500 Valby, Denmark, Department of Chemistry, Carlsberg Laboratory, Gamle Carlsbergvej 10, 2500 Valby, Denmark.
The primary structure of the acidic capsular antigen of Escherichia coli O9:K33:H- was shown by glycose analysis, methylation analysis, and by 1D and 2D 1H and 13C NMR spectroscopy to be composed of branched tetrasaccharide repeating units with the structure: [formula: see text]
Escherichia coli, capsular polysaccharide, NMR spectroscopy, pyruvate, K33 antigen
Structure type: polymer chemical repeating unit
Location inside paper: p.96, DPS
Compound class: K-antigen
Contained glycoepitopes: IEDB_115136,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152214,IEDB_174333,IEDB_190606,IEDB_423153,IEDB_983931,SB_192,SB_7,SB_86
Methods: methylation, NMR-2D, NMR
Comments, role: O-deacetylated capsular antigen
Related record ID(s): 7456
NCBI Taxonomy refs (TaxIDs): 1010797Reference(s) to other database(s): GTC:G56528VB, GlycomeDB:
27145
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,4 aDGlcp 99.51 73.02 81.12 68.82 72.73 61.29
4 bDGlcpA 104.45 75.46 76.88 78.70 78.39 174.91
3 aDGalp 101.27 69.61 70.22 70.58 72.20 62.59
aLFucp 100.23 69.40 75.56 81.22 68.44 16.17
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,4 aDGlcp 5.408 3.727 3.781 3.512 3.763 3.774-3.818
4 bDGlcpA 4.554 3.461 3.789 3.879 3.759 -
3 aDGalp 5.257 3.782 3.908 4.007 4.150 3.720-3.720
aLFucp 5.292 4.054 4.054 4.113 4.415 1.316
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,4 aDGlcp 99.51/5.408 73.02/3.727 81.12/3.781 68.82/3.512 72.73/3.763 61.29/3.774-3.818
4 bDGlcpA 104.45/4.554 75.46/3.461 76.88/3.789 78.70/3.879 78.39/3.759
3 aDGalp 101.27/5.257 69.61/3.782 70.22/3.908 70.58/4.007 72.20/4.150 62.59/3.720-3.720
aLFucp 100.23/5.292 69.40/4.054 75.56/4.054 81.22/4.113 68.44/4.415 16.17/1.316
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,4 | aDGlcp | 5.408 | 3.727 | 3.781 | 3.512 | 3.763 | 3.774
3.818 |
| 4 | bDGlcpA | 4.554 | 3.461 | 3.789 | 3.879 | 3.759 |
|
| 3 | aDGalp | 5.257 | 3.782 | 3.908 | 4.007 | 4.150 | 3.720
3.720 |
| | aLFucp | 5.292 | 4.054 | 4.054 | 4.113 | 4.415 | 1.316 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,4 | aDGlcp | 99.51 | 73.02 | 81.12 | 68.82 | 72.73 | 61.29 |
| 4 | bDGlcpA | 104.45 | 75.46 | 76.88 | 78.70 | 78.39 | 174.91 |
| 3 | aDGalp | 101.27 | 69.61 | 70.22 | 70.58 | 72.20 | 62.59 |
| | aLFucp | 100.23 | 69.40 | 75.56 | 81.22 | 68.44 | 16.17 |
|
There is only one chemically distinct structure:
Found 
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