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Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_130670,IEDB_133751,IEDB_135394,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_140529,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_150908,IEDB_151528,IEDB_151531,IEDB_179937,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_232584,IEDB_983931,SB_192,SB_7

• Article ID: 199
  Vinogradov EV, Van der Drift K, Thomas-Oates JE, Meshkov S, Brade H, Holst O "The structures of the carbohydrate backbones of the lipopolysaccharides from Escherichia coli rough mutants F470 (R1 core type) and F576 (R2 core type)" - European Journal of Biochemistry 261(3) (1999) 629-639
  

  The lipopolysaccharides (LPS) from Escherichia coli rough mutant strains F470 (R1 core type) and F576 (R2 core type) were deacylated yielding in each case a mixture of oligosaccharides with one predominant product which was isolated using high-performance anion-exchange chromatography. In addition, one oligosaccharide present in minor quantities was isolated from LPS of E. coli strain F576 (R2 core type). The structures of the oligosaccharides were determined by chemical analyses and NMR spectroscopic experiments. Furthermore, de-O-acylated and dephosphorylated LPS preparations were investigated by fast-atom bombardment and collision induced dissociation tandem mass spectrometry. The combined data allow us to deduce the following carbohydrate backbones of the E. coli R1 and R2 core types which share the following structure (Scheme 1 [see formula in text], but differ in the substituents R1 and R2 which for the R1 core type are predominantly: R1 = a-Gal-(1→2)-a-Gal-(1→2)-[b-Glc-(1→3)]a-Glc-(1→3)-a-Glc-, R2 = H and to a minor extent: R1 = a-Gal-(1→2)-a-Gal-(1→2)-[b-Glc-(1→3)]-a-Glc-(1→3)-a-Glc-, R2 = a-GlcN and for the R2 core type predominantly: R1 = a-GlcNAc-(1→2)-a-Glc-(1→2)-a-Glc-(1→3)-[a-Gal-(1→6)]-a-Glc-, R2 = H and to a minor extent: R1 = a-GlcNAc-[b-Gal-(1→4)]-(1→2)-a-Glc-(1→2)-a-Glc-[a-Gal-(1→6)]-(1→3)-a-Glc-, R2 = H in which all sugars are d-pyranoses (L,D-Hep, L-glycero-D-manno-heptopyranose; P, phosphate).

  Escherichia coli; lipopolysaccharide; core types R1 and R2; structural analysis

  NCBI PubMed ID: 10215878
  Journal NLM ID: 0107600
  Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
  Correspondence: oholst@fz-borstel.de
  Institutions: Center for Medicine and Biosciences, Borstel Research Center, Germany, Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research, Utrecht University, the Netherlands, Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia
  Methods: NMR-2D, NMR, chemical analysis, MS
  
   
  
  Escherichia coli F576
  CSDB ID 5696 (all data & tools)
• Article ID: 4304
  Gomery K, Müller-Loennies S, Brooks CL, Brade L, Kosma P, Di Padova F, Brade H, Evans SV "Antibody WN1 222-5 mimics Toll-like receptor 4 binding in the recognition of LPS" - Proceedings of the National Academy of Sciences of the USA 109(51) (2012) 20877-20882
  

  Escherichia coli infections, a leading cause of septic shock, remain a major threat to human health because of the fatal action to endotoxin (LPS). Therapeutic attempts to neutralize endotoxin currently focus on inhibiting the interaction of the toxic component lipid A with myeloid differentiating factor 2, which forms a trimeric complex together with Toll-like receptor 4 to induce immune cell activation. The 1.73-A resolution structure of the unique endotoxin-neutralizing protective antibody WN1 222-5 in complex with the core region shows that it recognizes LPS of all E. coli serovars in a manner similar to Toll-like receptor 4, revealing that protection can be achieved by targeting the inner core of LPS and that recognition of lipid A is not required. Such interference with Toll-like receptor complex formation opens new paths for antibody sepsis therapy independent of lipid A antagonists.

  structure, Escherichia coli, antibody, recognition, binding, Toll-like receptor 4

  NCBI PubMed ID: 23184990
  Publication DOI: 10.1073/pnas.1209253109
  Journal NLM ID: 7505876
  Publisher: National Academy of Sciences
  Correspondence: sml@fz-borstel.de; svevans@uvic.ca
  Institutions: Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada V8P 3P6, Research Center Borstel, Leibniz Center for Medicine and Biosciences, Medical and Biochemical Microbiology, D-23845 Borstel, Germany, Department of Biochemistry, Membrane Protein Disease Research Group, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada T6G 2H7, Department of Chemistry, University of Natural Resources and Life Sciences, A-1190 Vienna, Austria, Novartis Institutes for BioMedical Research, 4002 Basel, Switzerland
  Methods: ELISA, serological methods, crystallization
  
   
  
  Escherichia coli F576
  CSDB ID 27162 (all data & tools)