Taxonomic group: bacteria / Fusobacteria
(Phylum: Fusobacteria)
Host organism: Homo sapiens
Associated disease: periodontitis [ICD11:
DA0C 
];
infection due to Fusobacterium nucleatum [ICD11:
XN4P8 ]
The structure was elucidated in this paperNCBI PubMed ID: 33280822Publication DOI: 10.1016/j.carres.2020.108198Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov
nrc-cnrc.gc.ca
Institutions: Vaccine and Emerging Infections Research, Human Health Therapeutics Research Centre, National Research Council, Ottawa, ON, K1A 0R6, Canada
Fusobacterium nucleatum is a gram-negative bacterium, part of the normal human microflora. It is associated with various health complications, including periodontitis and colorectal cancer. Its surface is covered with lipopolysaccharide, which interacts with the immune system and can be involved in various processes in health and disease conditions. Here we present the results of structural analysis of core oligosaccharides from the lipopolysaccharides of several strains of F. nucleatum. Pure compounds were isolated using mild acid hydrolysis or alkaline deacylation of the lipopolysaccharides and analyzed by NMR spectroscopy, mass-spectrometry and chemical methods. All cores analyzed had a common octasaccharide region, including five heptose residues and a non-phosphorylated 3-deoxy-d-manno-oct-2-ulosonic acid residue. The common region is substituted with different additional components specific for each strain. By structure type the F. nucleatum core is similar to that produced by Aeromonas.
Lipopolysaccharide, NMR, structure, Fusobacterium nucleatum
Structure type: oligomer ; 712.4
Location inside paper: table 1, Fig.1, table S8, Hex1Hep2anhHexd1
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, 31P NMR, ESI-MS, mild acid hydrolysis, HPAEC, chemical methods, deamination, GPC, alkaline deacylation, fermentation
Comments, role: oligosaccharide was obtained by deamination of F. nucleatum strain 53G deacylated product; NMR temperature: 298 or 313.
Related record ID(s): 7582, 7583, 7584, 7585, 7586, 7587, 7588, 7590, 7591, 7592, 32207
NCBI Taxonomy refs (TaxIDs): 851
Show glycosyltransferases
NMR conditions: in D2O at 306 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6 C7
6,4,2 aXLLmanHepp 5.18 3.98 3.88 3.88 3.92 4.03 3.70-3.75
6,4 aDGalp 97.6 72.3 68.5 70.5 73.0 62.2
6 aXLLmanHepp 100.8 71.6 73.1 73.2 70.9 69.7 64.1
x?Suga 61.9 83.6 77.4 77.6 81.6 67.6
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6 H7
6,4,2 aXLLmanHepp 5.18 3.98 3.88 3.88 3.92 4.03 3.70-3.75
6,4 aDGalp 5.72 4.00 3.89 4.04 3.99 3.76-3.76
6 aXLLmanHepp 4.91 3.93 4.03 4.11 3.76 4.08 3.71-3.76
x?Suga 3.68-3.76 3.90 4.07 4.13 4.02 3.69-3.81
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 C7/H7
6,4,2 aXLLmanHepp 5.18/5.18 3.98/3.98 3.88/3.88 3.88/3.88 3.92/3.92 4.03/4.03 3.70-3.75/3.70-3.75
6,4 aDGalp 97.6/5.72 72.3/4.00 68.5/3.89 70.5/4.04 73.0/3.99 62.2/3.76-3.76
6 aXLLmanHepp 100.8/4.91 71.6/3.93 73.1/4.03 73.2/4.11 70.9/3.76 69.7/4.08 64.1/3.71-3.76
x?Suga 61.9/3.68-3.76 83.6/3.90 77.4/4.07 77.6/4.13 81.6/4.02 67.6/3.69-3.81
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 | H7 |
|---|
| 6,4,2 | aXLLmanHepp | 5.18 | 3.98 | 3.88 | 3.88 | 3.92 | 4.03 | 3.70
3.75 |
| 6,4 | aDGalp | 5.72 | 4.00 | 3.89 | 4.04 | 3.99 | 3.76
3.76 | |
| 6 | aXLLmanHepp | 4.91 | 3.93 | 4.03 | 4.11 | 3.76 | 4.08 | 3.71
3.76 |
| | x?Suga | 3.68
3.76 | 3.90 | 4.07 | 4.13 | 4.02 | 3.69
3.81 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 | C7 |
|---|
| 6,4,2 | aXLLmanHepp | 5.18 | 3.98 | 3.88 | 3.88 | 3.92 | 4.03 | 3.70
3.75 |
| 6,4 | aDGalp | 97.6 | 72.3 | 68.5 | 70.5 | 73.0 | 62.2 | |
| 6 | aXLLmanHepp | 100.8 | 71.6 | 73.1 | 73.2 | 70.9 | 69.7 | 64.1 |
| | x?Suga | 61.9 | 83.6 | 77.4 | 77.6 | 81.6 | 67.6 | |
|
There is only one chemically distinct structure:
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