Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: pneumonia [ICD11:
CA40 
];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
NCBI PubMed ID: 31606486Publication DOI: 10.1016/j.resmic.2019.10.001Journal NLM ID: 8907468Publisher: Elsevier
Correspondence: nikvol
obolensk.org; knirel
ioc.ac.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, 142279, Obolensk, Moscow Region, Russia
The Gram-negative opportunistic pathogen Klebsiella pneumoniae is a significant cause of community-acquired and healthcare-associated infections for which multidrug resistance is a concern worldwide. A major virulence determinant of K. pneumoniae is a polysaccharide capsule (CPS) which forms a barrier around the bacterial cell wall, providing protection from environmental pressures and immune responses of eukaryotic organisms. More than 70 chemical capsule structures of serologically typeable K. pneumoniae strains are known. However, there are little data on the CPS structure and cps gene cluster organization of clinical multidrug resistant K. pneumoniae strains. Our investigation of multidrug resistant carbapenemase OXA-48-producing K. pneumoniae strain KPB536 identified a capsular type that was structurally similar to K. pneumoniae K10 but different from any K. pneumoniae CPS reported so far. The content and organization of the cps gene cluster in K. pneumoniae KPB536 also was determined. The catalytic functions of glycosyltransferases coded by the cps_KPB536 gene cluster were assigned by comparison with those responsible for the synthesis of glycoside linkages in the CPSs of K. pneumoniae types K10 and K61.
capsular polysaccharide, glycosyltransferase, Klebsiella pneumoniae, multidrug resistance, Capsule biosynthesis genes
Structure type: polymer chemical repeating unit
Location inside paper: Fig.4, K61
Compound class: CPS, K-antigen
Contained glycoepitopes: IEDB_115136,IEDB_130701,IEDB_136906,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_423153,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_7,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, DNA techniques, acid hydrolysis, GPC, bioinformatics analysis
Related record ID(s): 7640, 7641, 32227
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G67194AM, GlycomeDB:
4930
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error