Heiss C, Wang Z, Thurlow CM, Hossain MJ, Sun D, Liles MR, Saper MA, Azadi P Structure of the capsule and lipopolysaccharide O-antigen from the channel catfish pathogen, Aeromonas hydrophila Carbohydrate Research486 (2019)
107858
The structure was elucidated in this paper NCBI PubMed ID:31683071 Publication DOI:10.1016/j.carres.2019.107858 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: C. Heiss <cheissuga.edu> Institutions: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA, 30602, USA, Department of Biological Sciences, Auburn University, 120 West Samford Avenue, Auburn, AL, 36849, USA, Department of Biological Chemistry, University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI, 48109, USA
A hypervirulent A. hydrophila (vAh) pathotype has been identified as the etiologic agent responsible for disease outbreaks in farmed carp species and channel catfish (Ictalurus punctatus) in China and the Southeastern United States, respectively. The possible route of infection has previously been unknown; however, virulence is believed to be multifactorial, involving the production/secretion of several virulence factors, including a high molecular weight group 4 capsular polysaccharide. Here we present chemical structural evidence of a novel capsule- and LPS-associated O-antigen found present in vAh isolated during these disease outbreaks. In this study, the chemical structure of the vAh O-antigen was determined by chemical analysis, Smith degradation, mass spectrometry, and 2D proton and carbon nuclear magnetic resonance (NMR) spectroscopy and found to be unique among described bacterial O-antigens. The O-antigen consists of hexasaccharide repeating units featuring a 4)-α-l-Fucp-(1-3)-β-d-GlcpNAc-(1-4)-α-l-Fucp-(1-4)-β-d-Glcp-(1- backbone, substituted with single residue side chains of α-d-Glcp and α-d-Quip3NAc linked to O-3 of the two fucose residues. The polysaccharide is partially O-acetylated on O-6 of the 4-substituted β-Glcp residue.
Lipopolysaccharide, NMR, polysaccharide structure, Aeromonas hydrophila, Fish pathogen, O-antigen capsule
Methods: 13C NMR, 1H NMR, gel filtration, NMR-2D, GC-MS, mild acid hydrolysis, GC, Smith degradation, MALDI-TOF MS, de-O-acetylation, composition analysis, SEC, HILIC Comments, role: deacetylated oligosaccharide from Smith degradation (OPS-SD).
Related record ID(s): 766, 767, 769, 770, 1026 NCBI Taxonomy refs (TaxIDs):1288394 Reference(s) to other database(s): GTC:G60951OU Show glycosyltransferases
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