Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 32902263Publication DOI: 10.1021/acs.analchem.0c02566Journal NLM ID: 0370536Correspondence: Alison J. Scott <ascott1
umaryland.edu>
Institutions: Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, Maryland 21201, United States, Structural Biology Core, NIDA IRP, NIH, 333 Cassell Drive, Room 1120, Baltimore, Maryland 21224, United States, Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine. Baltimore, Maryland 21205, United States, University of Gdansk, International Centre for Cancer Vaccine Science, Gdansk, Poland, Maastricht Multimodal Molecular Imaging (M4I) Institute, Maastricht University, Maastricht, The Netherlands
We developed a method to directly detect and map the Gram-negative bacterial virulence factor lipid A derived from lipopolysaccharide (LPS) by coupling acid hydrolysis with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). As the structure of lipid A (endotoxin) determines the innate immune outcome during infection, the ability to map its location within an infected organ or animal is needed to understand localized inflammatory responses that results during host-pathogen interactions. We previously demonstrated detection of free lipid A from infected tissue; however detection of lipid A derived from intact (smooth) LPS from host-pathogen MSI studies, proved elusive. Here, we detected LPS-derived lipid A from the Gram-negative pathogens, Escherichia coli (Ec, m/z 1797) and Pseudomonas aeruginosa (Pa, m/z 1446) using on-tissue acid hydrolysis to cleave the glycosidic linkage between the polysaccharide (core and O-antigen) and lipid A moieties of LPS. Using accurate mass methods, the ion corresponding to the major Ec and Pa lipid A species (m/z 1797 and 1446, respectively) were unambiguously discriminated from complex tissue substrates. Further, we evaluated potential delocalization and signal loss of other tissue lipids and found no evidence for either, making this LPS-to-Lipid A-MSI (LLA-MSI) method, compatible with simultaneous host-pathogen lipid imaging following acid hydrolysis. This spatially sensitive technique is the first step in mapping host-influenced de novo lipid A modifications, such as those associated with antimicrobial resistance phenotypes, during Gram-negative bacterial infection and will advance our understanding of the host-pathogen interface
Lipopolysaccharide, Pseudomonas aeruginosa, Escherichia coli, lipid A, Matrix-assisted laser desorption/ionization mass spectrometry
Structure type: oligomer ; 1797
Location inside paper: p.13669, Fig. 1(e), Fig. S1 (top)
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141807,IEDB_151531,IEDB_176772,IEDB_534864
Methods: acid hydrolysis, MALDI-TOF MS, HPLC
Comments, role: major structure lipid A
Related record ID(s): 32238
NCBI Taxonomy refs (TaxIDs): 1322345
Show glycosyltransferases
There is only one chemically distinct structure:
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