Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Shigella dysenteriae [ICD11:
XN285 
]
NCBI PubMed ID: 9742688Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: glau
helix.nih.gov
Institutions: National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
The O-specific polysaccharide (O-SP) of Shigella dysenteriae type 1 has been shown by others to have the structure →3)-α-L-Rhap-(1→3)-α-L-Rhap-(1→2)-α-D-Galp-(1→3)-α-D-GlcpNAc-(1→. We have shown in the past that IgM 3707 E9, an anti S. dysenteriae type 1 O-SP monoclonal antibody, binds specifically to the -α-L-Rhap-(1→2)-α-D-Galp-determinant of the polysaccharide. In this report we show that determinant to have hydrogen bonds, necessary for binding to the antibody, involving positions 3, 4 and 6 of the galactopyranosyl residue. The hydroxyl groups of the rhamnopyranosyl moiety of the immunodeterminant appear not to partake in hydrogen-bond interactions with the antibody. A model is presented of the Fv of IgM 3707 E9 based on our previously established cDNA-sequence and two known, highly homologous immunoglobulin crystal structures. The methyl glycoside of the immunodeterminant α-L-rhamnopyranosyl-(1→2)-α-D-galactopyranose is docked to the combining area of the Fv
deoxyfluoro sugars, ligand/antibody binding, protein structure
Structure type: polymer chemical repeating unit
Location inside paper: abstract
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_125611,IEDB_130668,IEDB_130669,IEDB_136105,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137483,IEDB_141794,IEDB_141807,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_225177,IEDB_885823,SB_7
Methods: DNA sequencing, NMR, spectrofluorometry
Biological activity: an anti S. dysenteriae type 1 O-SP monoclonal antibody, binds specifically to the aLRhap(1-2)aDGalp-determinant of the polysaccharide
3D data: conformation data
Related record ID(s): 2158, 3227, 3783, 6263, 6843, 20113, 22042, 23061, 124115, 130504
NCBI Taxonomy refs (TaxIDs): 984897Reference(s) to other database(s): GTC:G76746EO, GlycomeDB:
6223
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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