Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 11086692Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: mario.monteiro
nrc.ca
Institutions: Institute for Biological Sciences, National Research Council, 100 Sussex Dri6e, Ottawa, Ont., Canada K1A 0R6, Department of Pathobiology, Uni6ersity of Guelph, Guelph, Ont., Canada N1G 2W 1
The chemical and antigenic properties of the cell-surface lipopolysaccharides (LPSs) and capsular polysaccharides (CPSs) of seven representative strains of Actinobacillus suis from healthy and diseased pigs were investigated. Four strains produced a linear (1→6)-β-D-glucan homopolymer, β-D-Glcp-(1-[→6)-β-D-Glcp-(1-]n →, as a LPS-O-chain (O1) and as a CPS (K1). Polyclonal antisera prepared against a (1→6)-β-D-glucan-containing strain showed a positive reaction against both LPSs and CPSs derived from the above strains (designated serotype O1/K1). One strain carried the (1→6)-β-D-glucan solely as a LPS-O-chain (serotype O1) and two strains did not express the (1→6)-β-D-glucan, but, instead, produced a different O-chain (designated serotype 02); these three strains expressed their own characteristic CPSs. (1→6)-β-D-Glucan structures are common cell wall components of yeast, fungi and lichens, but, to our knowledge, this is the first time a (1→6)-β-D-glucan has been described in a prokaryotic organism. Conformational and nuclear magnetic resonance analyses showed that the β-D-Glcp-(1→6)-β-D-Glcp linkage was flexible and two distinct glycosidic conformers are described. Cross-reactive antibodies to the A. suis (1→6)-β-D-glucan could be detected in sera from a variety of species and in sera from specific pathogen free pigs. This cross-reactivity may arise from immuno-stimulation of organisms present in the surrounding environment that contain (1→6)-β-D-glucan, which may also explain the high incidence of false positive results in previous serological tests for A. suis. In addition, these (1→6)-β-D-glucan background antibodies may be protective against A. suis infection. The characterization herein of (1→6)-β-D-glucan is the foundation for the development of a serotyping system for A. suis.
Lipopolysaccharide, capsule, Actinobacillus suis, serotyping system, (1-6)-b-D-Glucan
Structure type: polymer chemical repeating unit
Trivial name: pustulan, β-1,6-glucan, β-(1,6)-glucan
Compound class: CPS, cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, sugar analysis, conformation analysis, GLC
Biosynthesis and genetic data: serological data
3D data: Monte Carlo conformational analysis
Related record ID(s): 45914, 47842, 47843, 48303, 48366, 48412, 48423, 48443, 49291, 50093
NCBI Taxonomy refs (TaxIDs): 716Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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