Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Pseudomonas aeruginosa [ICD11:
XN5L6 ]
The structure was elucidated in this paperNCBI PubMed ID: 33625828Publication DOI: 10.1021/acs.analchem.0c05069Journal NLM ID: 0370536Correspondence: c.bure
cbmn.u-bordeaux.fr
Institutions: Institute of Chemistry & Biology of Membranes & Nanoobjects, UMR 5248, CNRS, University of Bordeaux, Bordeaux INP, Bordeaux, Pessac F-33076, France, Department of Chemistry, University of Texas, Austin, Texas 78712, United States
Lipopolysaccharides (LPS) constitute the outermost layer of Gram-negative bacteria and consequently play an important role in bacterial infections. In order to address public health issues posed by Gram-negative bacteria, it is necessary to elucidate the structure of the molecular actors at the forefront of infections. LPS virulence and toxicity are partially modulated by lipid A, a hydrophobic saccharolipid that anchors LPS to the bacterial outer membrane. Understanding the lipid A structure is inherently intertwined with understanding its role as an endotoxin. Accordingly, several successful strategies incorporating tandem mass spectrometry have been applied toward the structural analysis of lipid A. Herein, a shotgun HCD strategy was applied toward the characterization of the lipid A profile of Pseudomonas aeruginosa PAO1. This analysis was enhanced by the development of an LC-MS/MS approach to eliminate isomeric signals in the MS/MS spectra that confounded characterization. Importantly, combining reverse phase chromatography with HCD and ultraviolet photodissociation analyses of the lipid A profile revealed the presence of previously unreported lipid A acyl chain positional isomers. Altogether, these strategies provide the most in-depth structural and molecular characterization of PAO1 lipid A to date.
characterization, Pseudomonas aeruginosa, lipid A, mass spectrometry
Structure type: oligomer ; 1353.8626 [M-H]-
Location inside paper: Fig. S2F
Compound class: lipid A
Contained glycoepitopes: IEDB_141807,IEDB_151531
Methods: acid hydrolysis, ESI-MS/MS, extraction, CID-MS, LC-MS/MS
Comments, role: pentaacylated lipid A
Related record ID(s): 7888, 7889, 7890, 7892, 10829
NCBI Taxonomy refs (TaxIDs): 208964
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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