Show legend Show as text

Structure type: polymer chemical repeating unit
Compound class: CPS, EPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136044,IEDB_136095,IEDB_137472,IEDB_141794,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88

• Article ID: 215
  Warnecke JM, Nitschke M, Moolenaar CEC, Rietschel ET, Hartmann RK, Mamat U "The 5'-proximal hairpin loop of lbi RNA is a key structural element in repression of D-galactan II biosynthesis in Klebsiella pneumoniae serotype O1" - Molecular Microbiology 36(3) (2000) 697-709
  

  The lbi (lipopolysaccharide biosynthesis interfering) RNA of phage Acm1, an untranslated RNA transcript of 97 nucleotides, previously shown to affect Opolysaccharide biosynthesis in various Escherichia coli strains, was found to downregulate the synthesis of the D-galactan II component of the O-specific polysaccharide in Klebsiella pneumoniae serotype O1. Enzymatic and Pb21 probing experiments revealed that lbi RNA consists of two consecutive stemloop structures, the 50-proximal hairpin loop of 15 nucleotides being particularly accessible to single strand-specific probes. Based on the assumption that the 50-proximal hairpin loop may be involved in an antisense interaction with cellular target RNAs, we randomly mutagenized one or two of its central nucleotides. Expression of mutated lbi RNA variants in K. pneumoniae serotype O1 relieved at least partly the repression of D-galactan II formation. In addition, a truncated version of lbi RNA lacking the 30-proximal hairpin loop was almost as efficient as the wild-type RNA in downregulating D-galactan II synthesis. The results obtained indicate that the 50-proximal hairpin loop of lbi RNA functions as a key structural element in the mechanism leading to the inhibition of D-galactan II biosynthesis in K. pneumoniae serotype O1.

  biosynthesis, structural, serotype, Klebsiella, Klebsiella pneumoniae, D-galactan, repression, RNA

  NCBI PubMed ID: 10844658
  Journal NLM ID: 8712028
  Publisher: Blackwell Publishing
  Correspondence: umamat@fz-borstel.de
  Institutions: Institute of Biochemistry, Department of Nephrology, Medical University of Lubeck, Ratzeburger Allee160, D-23538 Lubeck, Germany, Department of Immunochemistry and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany
  Methods: genetic methods
  
   
  
  Acetobacter methanolicus MB58/4
  CSDB ID 5728 (all data & tools)
• Article ID: 1778
  Knirel YA, Kochetkov NK "The structure of lipopolysaccharides of gram-negative bacteria. III. The structure of O-antigens: A review" - Biochemistry (Moscow) 59(12) (1994) 1325-1383
  

  This review summarizes data on the composition and structure of the O-antigens, the polysaccharide chains of the outer-membrane lipopolysaccharides (LPS) of Gram-negative bacteria defining the immunospecificity of these microbial cells. Special reference is given to some structural features of the O-antigens, such as the presence of unique monosaccharides and noncarbohydrate components, masked regularity, and the occurrence in one microorganism of LPS with structurally different polysaccharide chains. Antigenic relationships between microorganisms belonging to different taxonomic groups are discussed.

  structure, O-antigen, chemical composition, bacterial lipopolysaccharides, Salmonella livingstone C1

  NCBI PubMed ID: 7533007
  Journal NLM ID: 0376536
  Publisher: Nauka/Interperiodica
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
  
   
  
  Acetobacter methanolicus MB58/4, Acetobacter methanolicus MB70
  CSDB ID 108590 (all data & tools)
• Article ID: 2199
  Grimmecke HD, Mamat U, Lauk W, Shashkov AS, Knirel YA, Vinogradov EV, Kochetkov NK "Structure of the capsular polysaccharide and the O-side chain of the lipopolysaccharide from Acetobacter methanolicus MB 58/4 (IMET 10945), and of oligosaccharides resulting from their degradation by the bacteriophage Acm1" - Carbohydrate Research 220 (1991) 165-172
  

  The capsular polysaccharide (CPS) and the O-side-chain of the lipopolysaccharide (LPS) of Acetobacter methanolicus MB 58/4 (IMET 10945) have been shown to contain the same disaccharide repeating unit, namely, →2)-β-D-Galf-(1→3)-β-D-Galp-(1→. Degradation of the CPS and the LPS with the bacteriophage Acml gave fragments built up of 1-5 repeating units; the octasaccharide preponderated. The phage-associated depolymerase proved to be a β-D-galactofuranoside hydrolase.

  NCBI PubMed ID: 1811855
  Publication DOI: 10.1016/0008-6215(91)80014-e
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the U.S.S.R., Moscow, Institute of Biotechnology, Leipzig, Germany
  Methods: 13C NMR, 1H NMR, acid hydrolysis, alkaline degradation, phage degradation
  
   
  
  Acetobacter methanolicus MB58/4 (IMET 10945)
  CSDB ID 2665 (all data & tools)
• Article ID: 2291
  Grimmecke HD, Knirel YA, Shashkov AS, Kiesel B, Lauk W, Voges M "Structure of the capsular polysaccharide and the O-side-chain of the lipopolysaccharide from Acetobacter methanolicus MB 70, and of oligosaccharides resulting from their degradation by the bacteriophage Acm6" - Carbohydrate Research 253 (1994) 277-282
  

  Acetobacter methanolicus MB 70 was shown to be related to the type strain of this species MB 58/4 (IMET 10945) having the same galactan →2)-β-D-Galf-(1→3)-β-D-Galp-(1→ as the capsular polysaccharide (CPS) and the O-side-chain of the lipopolysaccharide (LPS). Additionally, a glucan built up of the disaccharide repeating unit →6)-α-D-Glcp-(1→2)-α-D-Glcp-(1→ was identified in strain MB 70. In the CPS, the polymers were present in the ratio approximately 1:1, whereas the glucan preponderated in the LPS. Bacteriophage Acm6 specific to A. methanolicus MB 70 hydrolysed selectively the glucan component of both CPS and LPS. Structural elucidation of the resulting oligosaccharides led to the identification of the phage-associated depolymerase as an endo-α-(1→6)-D-glucopyranoside hydrolase.

  NCBI PubMed ID: 7512445
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: Institute of Biotechnology, Leipzig, Germany
  
   
  
  Acetobacter methanolicus MB70
  CSDB ID 117631 (all data & tools)
• Article ID: 5197
  Pallach M, Marchetti R, Di Lorenzo F, Fabozzi A, Giraud E, Gully D, Paduano L, Molinaro A, D'Errico G, Silipo A "Zymomonas mobilis exopolysaccharide structure and role in high ethanol tolerance" - Carbohydrate Polymers 201 (2018) 293-299
  

  Bacterial cell surface exopolysaccharides (EPS) provide a protective barrier from the external milieu and participate in host-environment interactions. Zymomonas mobilis, an ethanologenic Gram negative bacterium, is used by the industry in bio-ethanol production process, due to its extraordinary resistance to a highly ethanolic environment. We found that Z. mobilis produces a mixture of two EPSs, an [α-(1→6)-D-Manp] mannose homopolymer and a galactose containing polysaccharide: [→2)-β-D-Galf-(1→3)-β-D-Galp-(1→]n. A physico-chemical study, conducted with diffusion-ordered spectroscopy (DOSY) and Dynamic Light Scattering (DLS), allowed to demonstrate that, differently from the majority of polysaccharides, ethanol is a good solvent for the galactose containing EPS, revealing that its chemical structure is specifically connected with the Zymomonas mobilis high ethanol tolerance.

  NMR spectroscopy, exopolysaccharide, DLS, DOSY, Ethanologenic bacteria, Zymomonas mobilis

  NCBI PubMed ID: 30241821
  Publication DOI: 10.1016/j.carbpol.2018.08.072
  Journal NLM ID: 8307156
  Publisher: Elsevier
  Correspondence: gerardino.derrico@unina.it; silipo@unina.it
  Institutions: Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Universita di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy, IRD, Laboratoire des Symbioses Tropicales et Mediterraneennes (LSTM), UMR IRD/ SupAgro/INRA/ UM2 /CIRAD, TA-A82/J - Campus de Baillarguet, 34398 Montpellier Cedex 5, France
  Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, SDS-PAGE, composition analysis, dynamic light scattering (DLS), DOSY
  
   
  
  Zymomonas mobilis
  CSDB ID 12753 (all data & tools)
• Article ID: 5791
  Knirel YA, Van Calsteren M "Bacterial exopolysaccharides" - Book: Comprehensive Glycoscience: From Chemistry to Systems Biology. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering (2021) 1-75
  

  Bacterial extracellular polysaccharides are known as a cell-bound capsule, a sheath, or a slime, which is excreted into the environment. They play an important role in virulence of medical bacteria and plant-to-symbiont interaction and are used for serotyping of bacteria and production of vaccines. Some exopolysaccharides have commercial applications in industry, and claims of health benefits have been documented for an increasing number of them. Exopolysaccharides have diverse composition and structure, and some contain sugar and non-sugar components that are found in bacterial carbohydrates only. The present article provides an updated collection of the data on exopolysaccharides of various classes of gram-negative and gram-positive bacteria reported until the end of 2019. When known, biosynthesis pathways of exopolysaccharides are treated in a summary manner. References are made to structure and biosynthesis relatedness between exopolysaccharides of different bacterial taxa as well as between bacterial polysaccharides and mammalian glycosaminoglycans.

  polysaccharide structure, Gram-negative bacteria, capsule, Biofilm, polysaccharide biosynthesis, gram-positive bacteria, Monosaccharide composition, Bacterial exopolysaccharide, non-sugar component

  Publication DOI: 10.1016/B978-0-12-819475-1.00005-5
  Publisher: Elsevier
  Correspondence: marie-rose.vancalsteren@canada.ca; yknirel@gmail.com
  Editors: Barchi J, Kamerling H
  Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food Canada, Saint-Hyacinthe, QC, Canada
  
   
  
  Zymomonas mobilis, Acidomonas methanolica MB 58/4, Acidomonas methanolica MB 70
  CSDB ID 6807 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: CPS
Contained glycoepitopes: IEDB_136044,IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88

• Article ID: 2199
  Grimmecke HD, Mamat U, Lauk W, Shashkov AS, Knirel YA, Vinogradov EV, Kochetkov NK "Structure of the capsular polysaccharide and the O-side chain of the lipopolysaccharide from Acetobacter methanolicus MB 58/4 (IMET 10945), and of oligosaccharides resulting from their degradation by the bacteriophage Acm1" - Carbohydrate Research 220 (1991) 165-172
  

  The capsular polysaccharide (CPS) and the O-side-chain of the lipopolysaccharide (LPS) of Acetobacter methanolicus MB 58/4 (IMET 10945) have been shown to contain the same disaccharide repeating unit, namely, →2)-β-D-Galf-(1→3)-β-D-Galp-(1→. Degradation of the CPS and the LPS with the bacteriophage Acml gave fragments built up of 1-5 repeating units; the octasaccharide preponderated. The phage-associated depolymerase proved to be a β-D-galactofuranoside hydrolase.

  NCBI PubMed ID: 1811855
  Publication DOI: 10.1016/0008-6215(91)80014-e
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the U.S.S.R., Moscow, Institute of Biotechnology, Leipzig, Germany
  Methods: 13C NMR, 1H NMR, acid hydrolysis, alkaline degradation, phage degradation
  
   
  
  Acetobacter methanolicus MB58/4 (IMET 10945)
  CSDB ID 116342 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: CPS
Contained glycoepitopes: IEDB_136044,IEDB_136095,IEDB_137472,IEDB_141794,IEDB_190606,IEDB_241097,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88

• Article ID: 2199
  Grimmecke HD, Mamat U, Lauk W, Shashkov AS, Knirel YA, Vinogradov EV, Kochetkov NK "Structure of the capsular polysaccharide and the O-side chain of the lipopolysaccharide from Acetobacter methanolicus MB 58/4 (IMET 10945), and of oligosaccharides resulting from their degradation by the bacteriophage Acm1" - Carbohydrate Research 220 (1991) 165-172
  

  The capsular polysaccharide (CPS) and the O-side-chain of the lipopolysaccharide (LPS) of Acetobacter methanolicus MB 58/4 (IMET 10945) have been shown to contain the same disaccharide repeating unit, namely, →2)-β-D-Galf-(1→3)-β-D-Galp-(1→. Degradation of the CPS and the LPS with the bacteriophage Acml gave fragments built up of 1-5 repeating units; the octasaccharide preponderated. The phage-associated depolymerase proved to be a β-D-galactofuranoside hydrolase.

  NCBI PubMed ID: 1811855
  Publication DOI: 10.1016/0008-6215(91)80014-e
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the U.S.S.R., Moscow, Institute of Biotechnology, Leipzig, Germany
  Methods: 13C NMR, 1H NMR, acid hydrolysis, alkaline degradation, phage degradation
  
   
  
  Acetobacter methanolicus MB58/4 (IMET 10945)
  CSDB ID 2664 (all data & tools)