Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
NCBI PubMed ID: 11015398Journal NLM ID: 7505564Publisher: American Society for Microbiology
Correspondence: macinnes
uoguelph.ca
Institutions: Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1, Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6
A cell surface antigen-typing system was devised for the swine pathogen Actinobacillus suis and used to examine the prevalence of different lipopolysaccharide (O) types in healthy and diseased pigs. The strains examined in this study were isolated from a variety of locations in Canada and from Kansas. Lipopolysaccharide preparations of 151 isolates of A. suis were characterized by immunoblotting using polyclonal antisera generated to strains SO4 (O1/K1), H89-1173 (O2/K3), and VSB 3714, a rough strain. Approximately 54% (62 of 114) of A. suis isolates from diseased pigs, all (11 of 11) isolates from healthy pigs, and all (4 of 4) reference strains reacted with O1/K1 antiserum. More than 80% (18 of 22) of A. suis strains used for bacterin production and approximately 41% (47 of 114) of isolates from diseased pigs bound O2/K3 antiserum. One isolate appeared to be rough, and five were untypeable. O1/K1- and O2/K3-reactive strains were equally prevalent in Kansas, whereas O2/K3-reactive strains were more common in Quebec and western Canada and O1/K1 strains were most common in Ontario. The fact that virtually all of the strains submitted for bacterin production were O2/K3-reactive strains is consistent with the notion that these strains may be more virulent than O1/K1 strains; alternatively, this may reflect geographic or other biases. In addition, we observed cross-reactivity between A. suis cell surface antigens and swine antisera to several other important pathogens. This finding may explain why previous attempts to develop a simple serodiagnostic test for A. suis have been unsuccessful.
serotype, Actinobacillus suis, Actinobacillus, Prevalence
Structure type: polymer chemical repeating unit
Location inside paper: p.3759
Trivial name: pustulan, β-1,6-glucan, β-(1,6)-glucan
Compound class: CPS, cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: serological methods
Biological activity: serological data
Related record ID(s): 45914, 47842, 47843, 48303, 48366, 48412, 48423, 48443, 49291, 50093
NCBI Taxonomy refs (TaxIDs): 716Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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