Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Clostridium perfringens [ICD11:
XN7J5 
]
The structure was elucidated in this paperNCBI PubMed ID: 9163894Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: cherniak
gsu.edu
Institutions: Department of Chemistry (LBCS), Georgia State University, Atlanta, GA, USA
The complete primary structure of the capsular polysaccharide of Clostridium perfringens Hobbs 5, an anaerobic bacterium implicated in food poisoning, was determined. The polysaccharide was isolated from C. perfringens Hobbs 5 cells, after deproteination, by ethanol precipitation and by ion-exchange chromatography. The polysaccharide was comprised of glucose, galactose, mannose, N-acetylglucosamine, N-acetylgalactosamine, and glucuronic acid, in equimolar ratios. Sequence and linkage assignments of the glycosyl residues were obtained by NMR spectroscopy, specifically by the combination of two-dimensional homonuclear TOCSY and NOESY experiments and heteronuclear (1H, 13C) multiple-quantum coherence (HMQC, HMQC-COSY, HMQC-TOCSY and HMBC) experiments. Thus, the envelope polysaccharide of C. perfringens Hobbs 5 was found to be a polymer composed of a hexasaccharide repeating unit with the following structure: [formula: see text] This structure is novel among bacterial cell-surface polysaccharides, and it is the first of many serotypically distinct capsular polysaccharides of C. perfringens to be described.
NMR, antigen, structure, capsular, polysaccharide, capsular polysaccharide, NMR spectroscopy, spectroscopy, 2D NMR spectroscopy, HMQC, Clostridium, Clostridium perfingens, TOCSY, NOESY
Structure type: polymer chemical repeating unit
Location inside paper: Abstract
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_131187,IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_137485,IEDB_140630,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_151527,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_190606,IEDB_423153,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_7,SB_72
Methods: NMR
NCBI Taxonomy refs (TaxIDs): 1502Reference(s) to other database(s): GTC:G76546LK, GlycomeDB:
25299
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error