Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 8759852Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: reeves
angis.su.oz.au
Institutions: Department of Microbiology, University of Sydney, New South Wales 2006, Australia
Colanic acid (CA) is an extracellular polysaccharide produced by most Escherichia coli strains as well as by other species of the family Enterobacteriaceae. We have determined the sequence of a 23-kb segment of the E. coli K-12 chromosome which includes the cluster of genes necessary for production of CA. The CA cluster comprises 19 genes. Two other sequenced genes (orf1.3 and galF), which are situated between the CA cluster and the O-antigen cluster, were shown to be unnecessary for CA production. The CA cluster includes genes for synthesis of GDP-L-fucose, one of the precursors of CA, and the gene for one of the enzymes in this pathway (GDP-D-mannose 4,6-dehydratase) was identified by biochemical assay. Six of the inferred proteins show sequence similarity to glycosyl transferases, and two others have sequence similarity to acetyl transferases. Another gene (wzx) is predicted to encode a protein with multiple transmembrane segments and may function in export of the CA repeat unit from the cytoplasm into the periplasm in a process analogous to O-unit export. The first three genes of the cluster are predicted to encode an outer membrane lipoprotein, a phosphatase, and an inner membrane protein with an ATP-binding domain. Since homologs of these genes are found in other extracellular polysaccharide gene clusters, they may have a common function, such as export of polysaccharide from the cell.
gene, polysaccharide, Escherichia, Escherichia coli, acid, cluster, gene cluster, extracellular polysaccharide, production, extracellular, organization, colanic acid
Structure type: polymer chemical repeating unit
Location inside paper: fig.1
Trivial name: colanic acid
Compound class: EPS
Contained glycoepitopes: IEDB_115015,IEDB_115136,IEDB_136044,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_142489,IEDB_146664,IEDB_149135,IEDB_151771,IEDB_190606,IEDB_423153,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_86,SB_88
Methods: genetic methods
Biosynthesis and genetic data: genetic data, biosynthetic data
NCBI Taxonomy refs (TaxIDs): 83333
Show glycosyltransferases
There is only one chemically distinct structure:
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