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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11: XN6P4

]

The structure was elucidated in this paper
NCBI PubMed ID: 7544238
Publication DOI: 10.1016/0008-6215(95)00041-Q
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: torgov

ioc.ac.ru
Institutions: Max-Planck-Institut für Immunbiologie, Freiburg, Germany

Structures for the N-acetylneuraminic acid (Neu5Ac)-containing O56 and O24 polysaccharides of Escherichia coli have been reported previously. During these studies unusual chemical shifts had been observed for the NMR signals for H-3eq and C-3 of the Neu5Ac residues of both polysaccharides. In further pursuing this phenomenon, we have reinvestigated the O56 and O24 polysaccharides as well as derived oligosaccharides by one- and two-dimensional NMR spectroscopy. The results showed that structures of both polysaccharides (PSs) had to be modified and formulated as [formula: see text] 2D ROESY spectra revealed a strong NOE between H-3eq of Neu5Ac and the protons of the side-chain sugar (H-3 and H-5 of α-D-Galp in the O56 PS and H-3 of α-D-Glcp in the O24 PS) and also between H-3ax of Neu5Ac and H-3 of β-D-Glcp in the main chain. This indicated a close spatial association of the seven-linked α-Neu5Ac and the side-chain residues α-D-Galp (O56 PS) and α-D-Glcp (O25 PS), respectively. The strong long-range spatial contacts caused the unusual chemical shifts of H-3eq and C-3 of Neu5Ac.

Escherichia coli, NMR spectroscopy, polysaccharide structure, 024 and 056 antigens

Structure type: oligomer
Location inside paper: p.86
Contained glycoepitopes: IEDB_130648,IEDB_136044,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_21,SB_25,SB_7,SB_88

Methods: NMR-2D, NMR, sugar analysis, Smith degradation

Related record ID(s): 326, 327, 328, 8382, 8411, 8412, 8413
NCBI Taxonomy refs (TaxIDs): 2170724
Reference(s) to other database(s): GTC:G39245NS, GlycomeDB:27253
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@@H](CO)[C@@H](O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O[C@@H]2O[C@H](CO)[C@H](O[C@@H]3O[C@H](CO)[C@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4NC(C)=O)[C@H](O)[C@H]3O)[C@H](O[C@]3(C(=O)O)C[C@H](O)[C@@H](N)[C@H]([C@H](O)[C@H](O)CO)O3)[C@H]2NC(C)=O)[C@H]1O)[C@@H](O)[C@H](O)CO

SVG MW SMILES 3D mol Ionize