Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
]
NCBI PubMed ID: 36436859Publication DOI: 10.1016/j.carbpol.2022.120318Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: pcescutti
units.it
Institutions: Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, DK-2200 Copenhagen, Denmark, GSK, Via Fiorentina 1, 53100 Siena, Italy, Department of Life Sciences, University of Trieste, via L. Giorgieri 1, Bdg. C11, 34127 Trieste, Italy
Burkholderia cenocepacia is an opportunistic pathogen isolated from cystic fibrosis patients where it causes infections that are extremely difficult to treat with antibiotics, and sometimes have a fatal outcome. Biofilm is a virulence trait of B. cenocepacia, and is associated with infection persistence and increased tolerance to antibiotics. In biofilms exopolysaccharides have an important role, conferring mechanical stability and antibiotic tolerance. Two different exopolysaccharides were isolated from B. cenocepacia H111 biofilms: a water-soluble polysaccharide rich in rhamnose and containing an L-Man residue, and a water-insoluble polymer made of glucose, galactose and mannose. In the present work, the product encoded by B. cenocepacia H111 bepA-L gene cluster was identified as the water-insoluble exopolysaccharide, using mutant strains and NMR spectroscopy of the purified polysaccharides. It was also demonstrated that the B. cenocepacia H111 wild type strain produces the water-insoluble exopolysaccharide in pellicles, thus underlining its potential importance in in vivo infections.
NMR spectroscopy, Exopolysaccharide structure, Biofilm, Burkholderia cenocepacia, bepA-L gene cluster
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1, H111-INS (= Bep)
Trivial name: water-insoluble EPS, Bep
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_7,SB_72
Methods: 1H NMR, sugar analysis, GLC, HR-MAS NMR, pellicles production, sequence analysis of gene clusters
Biosynthesis and genetic data: bepA-L
Comments, role: water-insoluble polysaccharide of the bepA-L gene cluster in B. cenocepacia H111 was named Bep
Related record ID(s): 20840, 20841, 20842
NCBI Taxonomy refs (TaxIDs): 1055524Reference(s) to other database(s): GTC:G54448EJ
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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