Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 36151499Publication DOI: 10.1007/978-1-0716-2581-1_12Journal NLM ID: 9214969Publisher: Springer
Correspondence: C. De Castro <decastro
unina.it>
Institutions: Department of Chemical Sciences, University of Naples, Naples, Italy, Department of Agricultural Sciences, University of Naples, Portici, Italy
Lipopolysaccharides (LPSs) are the main components of the external leaflet of the outer membrane of Gram-negative bacteria. They exert multiple functions, starting from conferring stability to the bacterial membrane to mediating the interaction of the microbe with the external environment. The composition and the structure of LPSs present tremendous diversity even within bacteria of the same species, and for this reason, the determination of the structure of these molecules is crucial because it can provide information on the motifs key for the virulence of a pathogen or that are associated to a bacterium of the commensal or beneficial microbiota. In addition, structural data disclose the effects triggered from a mutation or from the use of an antibiotic, or they can be used as tools to check the quality of adjuvants and/or medications, as vaccines, that make use of LPS. The structural study of LPSs is complex, and it can be achieved with the right combination of different techniques. In this frame, this chapter focuses on the two MS-based approaches, the gas chromatography-mass spectrometry (GC-MS) and the matrix-assisted laser desorption/ionization (MALDI).
carbohydrates, glycoconjugates, lipids, lipopolysaccharide (LPS), gas chromatography-mass spectrometry (GC-MS), matrix-assisted laser desorption/ionization (MALDI)
Structure type: oligomer ; 1796.2 [M-H]-
Location inside paper: Fig. 12(a), hexa-acylated lipid A
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141807,IEDB_151531,IEDB_176772,IEDB_534864
Methods: GC-MS, MALDI-TOF MS
Comments, role: methodology; the main lipid A from E. coli KG-279 LPS
NCBI Taxonomy refs (TaxIDs): 562
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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