Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia pseudomallei [ICD11:
XN3LD 
]
NCBI PubMed ID: 36402383Publication DOI: 10.1016/j.ijbiomac.2022.11.143Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: X. Mao <maoxh2012
hotmail.com>; J. Yan <zijie1011
yeah.net>
Institutions: Department of Clinical Microbiology and Immunology, College of Pharmacy and Medical Laboratory, Army Medical University (Third Military Medical University), Chongqing, China
O antigen is the major component of lipopolysaccharide LPS. The chemical structure of the O antigen determines the LPS serospecificity of the bacteria, and the diversity of O antigen is the basis for serotyping Burkholderia pseudomallei. In this study, structural elucidation of type B O antigen obtained from a clinical B. pseudomallei strain was conducted, and the effects of different types of LPS on macrophage differentiation were investigated. The O antigen was found to be composed of repeating units of [→4)-α-L-Rhap(1→4)-α-L-Rhap(1→2)-α-L-Rhap(1→2)-α-L-Rhap(1→3)-α-L-Rhap(1→3)-α-L-Rhap(1→4)-α-L-Rhap(1→6)-α-D-Galp(1→]n, where some of the →4)-α-L-Rhap(1 → units were substituted at O-3 by β-D-Xylp(1→ residues, and minor →3)-α-L-Rhap(1→ units were substituted at O-2 by β-D-Xylp(1→ residues. Meahwhile, the →6)-α-D-Galp(1→ units were substituted at O-3 by α-D-Galp(1→ residues. Furthermore, both type A and type B O antigens of B. pseudomallei could polarize macrophages toward the M1 phenotype, but the core oligosaccharides had no such activity. Therefore, we deduced that this polarization relies on the O antigen of LPS and might be related to the ability of B. pseudomallei to survive and replicate within macrophages. Thus, the characterization of different types of O antigen structural motifs is essential for further clarifying the persistence/survival mechanisms and inflammatory potential of B. pseudomallei.
O-antigen, B.pseudomallei, Macrophage polarization
Structure type: polymer chemical repeating unit
Location inside paper: p. 804
Trivial name: type A OPS
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, GC-MS, SDS-PAGE, composition analysis, HPLC, statistical analysis, mild acid degradation, flow cytometry analysis, HPGPC, measurement of cytokines, qRT-PCR, nitric oxide assay, macrophage polarization, deletion of wbiI
Comments, role: Burkholderia pseudomallei strain BPC006 carried LPS genotype A
Related record ID(s): 21008
NCBI Taxonomy refs (TaxIDs): 28450,
1229785
Show glycosyltransferases
There is only one chemically distinct structure:
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