Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
];
infection due to Pseudomonas aeruginosa [ICD11:
XN5L6 ]
NCBI PubMed ID: 35674342Publication DOI: 10.1021/acsinfecdis.2c00184Journal NLM ID: 101654580Publisher: Washington, DC: American Chemical Society
Correspondence: J. Sauvageau <janelle.sauvageau
nrc-cnrc.gc.ca>
Institutions: Vaccine and Emerging Infections Research, Human Health Therapeutics Research Centre, National Research Council, Ottawa, ON K1A 0R6, Canada
Pseudomonas aeruginosa was added to the World Health Organization's priority pathogen list for research and development of new antibiotics in 2017. Alongside the development of new antibiotics to fight antimicrobial-resistant P. aeruginosa, vaccines would be an appealing addition to the toolbox health professionals have against this bacteria, which causes life-threatening respiratory infections. Recently, the structure of a novel immunogenic terminal carbohydrate moiety on the cell surface of P. aeruginosa was elucidated, consisting of a 3-O-methyl(1→4)-α-D-rhamnan pentasaccharide. As isolating this oligosaccharide from P. aeruginosa in sufficient amounts for producing a conjugate vaccine is challenging, herein we describe the synthesis of 3-O-methyl D-rhamnose oligosaccharide. We also report the conjugation of the synthetic pentasaccharide to human serum albumin and its resulting immunogenicity.
synthesis, D-mannose, Pseudomonas aeruginosa, vaccines, D-rhamnose, Conjugation, rhamnose pentasaccharide
Structure type: fragment of a bigger structure
Location inside paper: p. 1337, Fig. 1
Trivial name: A-band polysaccharide (A-PS)
Compound class: CPA
Contained glycoepitopes: IEDB_130701,IEDB_1394181,IEDB_144983,IEDB_152206,IEDB_983930,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, 31P NMR, ESI-MS, Western blotting, chemical synthesis, chemical methods, immunization, conjugation
Comments, role: the non-reducing end of the A-band polysaccharide (A-PS) after periodate oxidation, subsequent reduction, and a second oxidation.
NCBI Taxonomy refs (TaxIDs): 208964
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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