Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Cyprinus carpio
Associated disease: motile aeromonad septicemia (MAS)
The structure was elucidated in this paperNCBI PubMed ID: 35717683Publication DOI: 10.1016/j.carres.2022.108602Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: A. Turska-Szewczuk <aturska
hektor.umcs.lublin.pl>
Institutions: Department of Genetics and Microbiology, Institute of Biological Sciences, M. Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland
The structure of the O-specific polysaccharide (OPS) from Aeromonas encheleia strain A4 lipopolysaccharide was investigated. A. encheleia strain A4, classified into the new provisional serogroup PGO1 predominating among aeromonads in Polish aquaculture, was isolated from common carp tissues during an outbreak of MAI/MAS disease on a fish farm. The high-molecular-weight OPS fraction liberated from the lipopolysaccharide after mild acid hydrolysis followed by gel-permeation chromatography was studied with chemical methods, mass spectrometry, and one- and two-dimensional 1H and 13C NMR spectroscopy techniques. Inter-residue correlations were identified in 1H,13C-heteronuclear multiple-bond correlation (HMBC) and 1H,1H NOESY experiments. It was found that the O-specific polysaccharide of A. encheleia strain A4 consists of branched pentasaccharide repeating units with the following structure:→2)[α-d-Fucp3NRHb-(1→3)]-α-l-Rhap-(1→3)-β-l-Rhap-(1→4)-α-l-Rhap-(1→3)-β-d-QuipNAc-(1→.
Lipopolysaccharide, O-antigen, NMR spectroscopy, O-specific polysaccharide, Aeromonas encheleia, Fucp3N
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1, p. 108602-5
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136105,IEDB_225177,IEDB_885823
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, SDS-PAGE, sugar analysis, GLC, enzymatic degradation, GPC, extraction, isolation, cultivation
NCBI Taxonomy refs (TaxIDs): 73010
Show glycosyltransferases
NMR conditions: in D2O at 305 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,3,3,3 lR3HOBut 175.2 46.0 66.1 23.1
3,4,3,3 aDFucp3N 96.1 66.8 52.2 71.8 67.9 16.3
3,4,3 aLRhap 102.4 76.0 75.5 72.0 70.4 17.8
3,4 bLRhap 101.4 71.6 81.8 72.2 73.2 17.8
3 aLRhap 102.3 71.2 70.2 83.6 68.4 17.5
2 Ac 175.4 23.6
bDQuipN 102.6 56.7 82.6 74.8 73.2 17.9
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,3,3,3 lR3HOBut - 2.52 4.25 1.27
3,4,3,3 aDFucp3N 5.17 4.03 4.42 3.79 4.51 1.21
3,4,3 aLRhap 5.17 4.37 4.02 3.41 3.91 1.30
3,4 bLRhap 4.76 4.16 3.69 3.51 3.51 1.36
3 aLRhap 4.82 3.86 3.85 3.58 4.08 1.26
2 Ac - 2.15
bDQuipN 4.89 3.85 3.38 3.26 3.45 1.31
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,3,3,3 lR3HOBut 46.0/2.52 66.1/4.25 23.1/1.27
3,4,3,3 aDFucp3N 96.1/5.17 66.8/4.03 52.2/4.42 71.8/3.79 67.9/4.51 16.3/1.21
3,4,3 aLRhap 102.4/5.17 76.0/4.37 75.5/4.02 72.0/3.41 70.4/3.91 17.8/1.30
3,4 bLRhap 101.4/4.76 71.6/4.16 81.8/3.69 72.2/3.51 73.2/3.51 17.8/1.36
3 aLRhap 102.3/4.82 71.2/3.86 70.2/3.85 83.6/3.58 68.4/4.08 17.5/1.26
2 Ac 23.6/2.15
bDQuipN 102.6/4.89 56.7/3.85 82.6/3.38 74.8/3.26 73.2/3.45 17.9/1.31
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,3,3,3 | lR3HOBut |
| 2.52 | 4.25 | 1.27 | |
| 3,4,3,3 | aDFucp3N | 5.17 | 4.03 | 4.42 | 3.79 | 4.51 | 1.21 |
| 3,4,3 | aLRhap | 5.17 | 4.37 | 4.02 | 3.41 | 3.91 | 1.30 |
| 3,4 | bLRhap | 4.76 | 4.16 | 3.69 | 3.51 | 3.51 | 1.36 |
| 3 | aLRhap | 4.82 | 3.86 | 3.85 | 3.58 | 4.08 | 1.26 |
| 2 | Ac |
| 2.15 | |
| | bDQuipN | 4.89 | 3.85 | 3.38 | 3.26 | 3.45 | 1.31 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,3,3,3 | lR3HOBut | 175.2 | 46.0 | 66.1 | 23.1 | |
| 3,4,3,3 | aDFucp3N | 96.1 | 66.8 | 52.2 | 71.8 | 67.9 | 16.3 |
| 3,4,3 | aLRhap | 102.4 | 76.0 | 75.5 | 72.0 | 70.4 | 17.8 |
| 3,4 | bLRhap | 101.4 | 71.6 | 81.8 | 72.2 | 73.2 | 17.8 |
| 3 | aLRhap | 102.3 | 71.2 | 70.2 | 83.6 | 68.4 | 17.5 |
| 2 | Ac | 175.4 | 23.6 | |
| | bDQuipN | 102.6 | 56.7 | 82.6 | 74.8 | 73.2 | 17.9 |
|
There is only one chemically distinct structure:
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