Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 10844668Journal NLM ID: 8712028Publisher: Blackwell Publishing
Correspondence: president
aac.pref.aichi.jp
Institutions: Department of Bacteriology, Nagoya University School of Medicine, Nagoya, Japan
Analyses of crystals of Escherichia coli Re lipopolysaccharide (LPS) formed after storage in 1% triethylamine indicate that the LPS molecules are assembled to form a monolayered structure consisting of a novel heterogeneous lattice structure, the greater part of which is occupied by one kind of lattice (lattice I), corresponding to the acyl chain portion of lipid A, and the remainder is occupied by the other kind of lattice (lattice II), corresponding to the 3-deoxy-Dmanno-octulosonic acid (dOclA) dimer and the N-acetylglucosamine disaccharide of lipid A. X-ray diffraction reveals that the type of cell is monoclinic (a = 5.53 A, b = 27.2 A, c = 6.47 A, α = 90 degrees, β = 125.8 degrees, γ = 90 degrees). Atomic force microscopy shows that crystals consist of multiple layers; the thickness of a layer corresponds to the b-axis value, and two types of surface topographies are visualized. One, regarded as the view onto the acyl chain ends, is two-dimensional arrays of oval bodies that constitute the lattice, with the lattice constants corresponding to the a- and c-axes and the angle of β (lattice I). The other, regarded as the view onto the dOclA dimers, is two-dimensional arrays of dromedary-back-like bodies that constitute the lattice with axes of 9.0 and 10.7 A and the angle of 65 degrees formed by both axes (lattice II). Based on these results, we present the molecular model of E. coli Re LPS.
Lipopolysaccharide, structure, Bacterial, molecular, Molecular Structure, lattice, Escherichia, Escherichia coli, endotoxin, formation, Re, implication
Structure type: oligomer
Aglycon: lipid A
Trivial name: outer core region
Compound class: core oligosaccharide, LOS, LPS
Contained glycoepitopes: IEDB_130650,IEDB_130659
Methods: X-ray, atomic force microscopy
3D data: lattice structure
Related record ID(s): 13, 829, 875, 924, 6252, 6306, 6431, 7184, 8403, 10584, 10590, 23706, 24940
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G36348JQ, GlycomeDB:
5576
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error