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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11: XN6P4

]

NCBI PubMed ID: 35093846
Publication DOI: 10.1016/j.cis.2022.102603
Journal NLM ID: 8706645
Publisher: Amsterdam: Elsevier
Correspondence: N. Paracini <nicolo.paracini

mau.se; S. Micciulla <micciulla

ill.fr>
Institutions: Malmö University, Malmö, Sweden, Physics Departent, Technische Universität Darmstadt, Darmstadt, Germany, Université Grenoble Alpes, CNRS, CERMAV, Grenoble, France, Institut Laue-Langevin, Grenoble, France

Lipopolysaccharides (LPSs) are a constitutive element of the cell envelope of Gram-negative bacteria, representing the main lipid in the external leaflet of their outer membrane (OM) lipid bilayer. These unique surface-exposed glycolipids play a central role in the interactions of Gram-negative organisms with their surrounding environment and represent a key element for protection against antimicrobials and the development of antibiotic resistance. The biophysical investigation of a wide range of different types of in vitro model membranes containing reconstituted LPS has revealed functional and structural properties of these peculiar membrane lipids, providing molecular-level details of their interaction with antimicrobial compounds. LPS assemblies reconstituted at interfaces represent a versatile tool to study the properties of the Gram-negative OM by exploiting several surface-sensitive techniques, in particular X-ray and neutron scattering, which can probe the structure of thin films with sub-nanometer resolution. This review provides an overview of different approaches employed to investigate structural and biophysical properties of LPS, focusing on studies on Langmuir monolayers of LPS at the air/liquid interface and a range of supported LPS-containing model membranes reconstituted at solid/liquid interfaces.

lipopolysaccharides, outer membrane, antimicrobial peptides, model bacterial interfaces, X-ray and neutron scattering

Structure type: oligomer
Location inside paper: Fig. 2C, E. coli R1
Aglycon: (->3) HepI+KdoI+Lipid A
Trivial name: outer core oligosaccharide
Compound class: core oligosaccharide, LPS
Contained glycoepitopes: IEDB_131186,IEDB_135818,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_153543,IEDB_190606,IEDB_2189047,IEDB_983931,SB_192,SB_7

Comments, role: review

NCBI Taxonomy refs (TaxIDs): 562
Show glycosyltransferases

There is only one chemically distinct structure:

OC[C@H](O)[C@H]1O[C@H](O)[C@@H](O)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H]3O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)[C@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize