Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Enterobacter cloacae [ICD11:
XN3YM 
]
The structure was elucidated in this paperNCBI PubMed ID: 35779303Publication DOI: 10.1016/j.carres.2022.108612Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: A.V. Perepelov <andreivperepelov
gmail.com>
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China, Department of Colorectal Surgery, Tianjin Union Medical Center, Nankai University, Tianjin, China
The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2559 and studied by sugar analysis along with 1D and 2D 1H and 13C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established. The O-antigen gene cluster of Enterobacter cloacae G2559 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-antigen structure.
Lipopolysaccharide, NMR, O-antigen, bacterial polysaccharide structure, O-antigen gene cluster, Enterobacter cloacae
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137485,IEDB_140116,IEDB_141830,IEDB_142488,IEDB_143632,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_983930,IEDB_983931,SB_136,SB_192,SB_196,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, sugar analysis, GLC, GPC, mild acid degradation, delipidation, dialysis, sequencing, function analysis of gene cluster
NCBI Taxonomy refs (TaxIDs): 550Reference(s) to other database(s): GTC:G05121PY
Show glycosyltransferases
NMR conditions: in D2O at 326 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2,2 aDManp 104.0 71.3 71.7 67.7 72.9 66.2
3,2,4 aDGlcp 100.9 73.6 74.3 70.9 73.8 62.1
3,2 aDManp 100.3 80.0 72.0 75.2 72.0 61.6
3 bDManp 102.6 76.3 75.3 68.3 78.3 62.3
2 Ac 175.4 23.6
aDFucpN 98.5 50.2 78.0 72.5 68.1 16.7
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2,2 aDManp 5.02 4.14 3.87 3.95 3.82 3.50-4.06
3,2,4 aDGlcp 5.37 3.57 3.72 3.42 3.78 3.79-3.88
3,2 aDManp 5.35 4.05 4.29 3.98 4.22 3.83-3.83
3 bDManp 4.81 4.00 3.72 3.62 3.39 3.73-3.92
2 Ac - 2.04
aDFucpN 4.83 4.24 4.07 3.97 4.14 1.23
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2,2 aDManp 104.0/5.02 71.3/4.14 71.7/3.87 67.7/3.95 72.9/3.82 66.2/3.50-4.06
3,2,4 aDGlcp 100.9/5.37 73.6/3.57 74.3/3.72 70.9/3.42 73.8/3.78 62.1/3.79-3.88
3,2 aDManp 100.3/5.35 80.0/4.05 72.0/4.29 75.2/3.98 72.0/4.22 61.6/3.83-3.83
3 bDManp 102.6/4.81 76.3/4.00 75.3/3.72 68.3/3.62 78.3/3.39 62.3/3.73-3.92
2 Ac 23.6/2.04
aDFucpN 98.5/4.83 50.2/4.24 78.0/4.07 72.5/3.97 68.1/4.14 16.7/1.23
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2,2 | aDManp | 5.02 | 4.14 | 3.87 | 3.95 | 3.82 | 3.50
4.06 |
| 3,2,4 | aDGlcp | 5.37 | 3.57 | 3.72 | 3.42 | 3.78 | 3.79
3.88 |
| 3,2 | aDManp | 5.35 | 4.05 | 4.29 | 3.98 | 4.22 | 3.83
3.83 |
| 3 | bDManp | 4.81 | 4.00 | 3.72 | 3.62 | 3.39 | 3.73
3.92 |
| 2 | Ac |
| 2.04 | |
| | aDFucpN | 4.83 | 4.24 | 4.07 | 3.97 | 4.14 | 1.23 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2,2 | aDManp | 104.0 | 71.3 | 71.7 | 67.7 | 72.9 | 66.2 |
| 3,2,4 | aDGlcp | 100.9 | 73.6 | 74.3 | 70.9 | 73.8 | 62.1 |
| 3,2 | aDManp | 100.3 | 80.0 | 72.0 | 75.2 | 72.0 | 61.6 |
| 3 | bDManp | 102.6 | 76.3 | 75.3 | 68.3 | 78.3 | 62.3 |
| 2 | Ac | 175.4 | 23.6 | |
| | aDFucpN | 98.5 | 50.2 | 78.0 | 72.5 | 68.1 | 16.7 |
|
There is only one chemically distinct structure:
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