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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11: XN6P4

]

NCBI PubMed ID: 12973815
Journal NLM ID: 8204476
Publisher: Wiley-VCH
Correspondence: volpi

unimo.it
Institutions: Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy

A rapid, highly sensitive and reproducible high-performance capillary electrophoresis (HPCE) method (electrokinetic chromatography with sodium dodecyl sulfate) is described for the determination of the lipopolysaccharide (LPS) and detoxified LPS (D-LPS), produced by both alkaline treatment in anhydrous conditions and mild acid hydrolysis, from Escherichia coli 055:B5 bacteria. LPS and D-LPS are separated and readily determined within 25 min on an uncoated fused-silica capillary using normal polarity at 20 kV and detection at 200 nm. A linear relationship (correlation coefficient greater than about 0.97) was found for the LPS and the two D-LPS species over a wide range of concentrations, from approximately 120 to 360 ng, with a detection sensitivity less than about 100 ng. Furthermore, HPCE was able to separate several molecular species mainly due to the presence of populations with O-specific polysaccharides of distinct and increasing mean chain lengths. This approach could be of great importance for the quantitative determination of LPS and D-LPS during the purification and preparation processes, also considering the importance of D-LPS in the preparation of human vaccines, and for the qualitative evaluation of the heterogeneity of LPS and the O-polysaccharide components

Lipopolysaccharide, Escherichia, Escherichia coli, separation, capillary electrophoresis, Detoxified lipopolysaccharide

Structure type: suggested polymer biological repeating unit
Location inside paper: p. 3102
Aglycon: core
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_136044,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_1391962,IEDB_141794,IEDB_141807,IEDB_142078,IEDB_143794,IEDB_144990,IEDB_150899,IEDB_151528,IEDB_151531,IEDB_167071,IEDB_190606,SB_137,SB_156,SB_165,SB_166,SB_173,SB_187,SB_195,SB_29,SB_7,SB_88

Methods: SDS-PAGE, mild acid hydrolysis, alkaline degradation, HPCE
Comments, role: chemical repeat frame is different in the paper

Related record ID(s): 5554, 7239, 11273, 20658, 28398, 30374, 108629, 113595, 113759
NCBI Taxonomy refs (TaxIDs): 2170726
Reference(s) to other database(s): GTC:G96674TM
Show glycosyltransferases

There are 2 chemically distinct structures. Please, select:

*OC[C@H]1O[C@@H](O[C@H]2[C@@H](O)[C@@H](CO)O[C@H](O[C@H]3[C@@H](O)[C@@H](CO)O[C@@H](*)[C@@H]3NC(C)=O)[C@@H]2O)[C@H](NC(C)=O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O[C@@H]2O[C@@H](C)[C@@H](O)C[C@@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize