Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 9673232Journal NLM ID: 0246127Publisher: American Society for Microbiology
Correspondence: reeves
angis.su.oz.au
Institutions: Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia
The O157:H7 clone of Escherichia coli, which causes major, often prolonged outbreaks of gastroenteritis with hemolytic-uremic syndrome (HUS) such as those in Japan, Scotland, and the United States recently, is thought to be resident normally in cattle or other domestic animals. This clone is of major significance for public health and the food industry. We have developed a fast method for sequencing a given O antigen gene cluster and applied it to O157. The O157 O antigen gene cluster is 14 kb in length, comprising 12 genes and a remnant H-repeat unit. Based on sequence similarity, we have identified all the necessary O antigen genes, including five sugar biosynthetic pathway genes, four transferase genes, the O unit flippase gene, and the O antigen polymerase gene. By PCR testing against all 166 E. coli O serogroups and a range of gram-negative bacterial strains, including some that cross-react serologically with E. coli O157 antisera, we have found that certain O antigen genes are highly specific to O157 E. coli. This work provides the basis for a sensitive test for rapid detection of O157 E. coli. This is important both for decisions on patient care, since early treatment may reduce the risk of life-threatening complications, and for detection of sources of contamination. The method for fast sequencing of O antigen gene clusters plus an ability to predict which genes will be O antigen specific will enable PCR tests to be developed as needed for other clones of E. coli or, once flanking genes are identified, clones of any gram-negative bacterium
antigen, O-antigen, Escherichia, Escherichia coli O157, gene cluster
Structure type: suggested polymer biological repeating unit
Location inside paper: Fig. 2
Aglycon: core
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_136045,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_146664,IEDB_152214,IEDB_174333,IEDB_885822,IEDB_983931,SB_192,SB_86
Methods: PCR, DNA sequencing
Biosynthesis and genetic data: biosynthesis data, genetic data
Related record ID(s): 1619, 7417, 8701, 8702, 8988, 20690, 27347, 30269, 30358, 105778, 108677
NCBI Taxonomy refs (TaxIDs): 1045010Reference(s) to other database(s): GTC:G60704LX, GlycomeDB:
27267
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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